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Gene Expression Data
Assay Details
Assay
Reference: J:319706 Silachev D, et al., Mouse models characterize GNAO1 encephalopathy as a neurodevelopmental disorder leading to motor anomalies: from a severe G203R to a milder C215Y mutation. Acta Neuropathol Commun. 2022 Jan 28;10(1):9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7425406
Gene symbol: Nes
Gene name: nestin
Antibody: anti-Nestin (2Q178; ab6142)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4A: embryonic day 18.5 (more )
Note: Double labeled: green - Nes; red - Eomes (assay MGI:7425415).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex ventricular zone forebrain radial glial cell Present 4A Merged , 4A Nestin
Specimen 4B: embryonic day 18.5; Gnao1em2Katv/Gnao1+ (more )
Note: Double labeled: green - Nes; red - Eomes (assay MGI:7425415).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex ventricular zone forebrain radial glial cell Present 4B Nestin , 4B Merged The intensity of Nestin immunostaining was unchanged compared with wild type. There was a reduction in thickness of the layer, which indicated a decrease in the total number of radial glial cells.
Specimen 4C: embryonic day 18.5; Gnao1em2Katv/Gnao1em2Katv (more )
Note: Double labeled: green - Nes; red - Eomes (assay MGI:7425415).
Structure Cell Type Level Pattern Image Note
TS26: cerebral cortex ventricular zone forebrain radial glial cell Present 4C Nestin , 4C Merged The intensity of Nestin immunostaining was unchanged compared with wild type. There was a reduction in thickness of the layer, which indicated a decrease in the total number of radial glial cells.
Specimen S6C +/+: embryonic day 18.5 (more )
Structure Cell Type Level Pattern Image Note
TS26: frontal cortex forebrain radial glial cell Present Regionally restricted S6C +/+ Nestin Expression was detected in radial glial cells in the motor cortex.
Specimen S6C G203R/+: embryonic day 18.5; Gnao1em1Katv/Gnao1+ (more )
Structure Cell Type Level Pattern Image Note
TS26: frontal cortex forebrain radial glial cell Present Regionally restricted S6C G203R/+ Nestin Expression was detected in radial glial cells in the motor cortex. No change in staining intensity was observed compared with wild type.
Specimen S6C G203R/G203R: embryonic day 18.5; Gnao1em1Katv/Gnao1em1Katv (more )
Structure Cell Type Level Pattern Image Note
TS26: frontal cortex forebrain radial glial cell Present Regionally restricted S6C G203R/G203R Nestin Expression was detected in radial glial cells in the motor cortex. No change in staining intensity was observed compared with wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory