Gene Expression Data
Assay Details
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Assay
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Reference:
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J:330271
Tekko T, et al., Assessing Myf5 and Lbx1 contribution to carapace development by reproducing their turtle-specific signatures in mouse embryos. Dev Dyn. 2022 Oct;251(10):1698-1710
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7423788
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Probe:
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Pax3 probe59
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A/A':
embryonic day 10.5
(more  )
Specimen
5A/A':
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
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5A
,
5A'
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Stronger expression in epithelial hypaxial lip.
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Specimen
5B/B':
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Myf5em2Mllo/Myf5em2Mllo
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
Widespread |
5B'
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5B
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Expressed throughout the dermomyotome, but its hypaxial pattern was slightly different from that observed in control embryos.
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Specimen
5C/C':
embryonic day 10.5
(more )
Specimen
5C/C':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
5D/D':
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Myf5em2Mllo/Myf5em2Mllo
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
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5D
,
5D'
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The hypaxial dermomyotomal lip showed less epithelial morphology than in wild-type.
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Specimen
7I:
embryonic day 10.5
(more )
Specimen
7I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
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7I
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Stronger expression in epithelial hypaxial lip.
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Specimen
7I':
embryonic day 10.5
(more )
Specimen
7I':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
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7I'
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Stronger expression in epithelial hypaxial lip.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: FVB/NJ
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Mutant Allele(s):
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Myf5em3Mllo/Myf5em3Mllo
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dermomyotome |
Present |
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The hypaxial dermomyotomal lip showed less epithelial morphology than in wild-type.
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Analysis Tools