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Gene Expression Data
Assay Details
Assay
Reference: J:179038 Baston-Buest DM, et al., In vitro culture does not alter the expression of vascular endothelial growth factor and its receptors in single murine preimplantation embryos. Gynecol Obstet Invest. 2011;71(1):24-31
Assay type: RT-PCR
MGI Accession ID: MGI:7423611
Gene symbol: Vegfa
Gene name: vascular endothelial growth factor A
Probe: Vegfa-pO1, Vegfa-pP1
Visualized with: Ethidium bromide
Assay notes: Two rounds of nested PCR were used; the first set of primers was (MGI:7423596) and the second set was (MGI:7423597).
Results Image: 2a
 Sample Information Bands Other Sample Information
Lane AgeStructure 188 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
M Mol. Wt. Marker Lane
n Control
1 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
2 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
3 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
4 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
5 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
6 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
7 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
8 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
9 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
10 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
11 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
12 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
13 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
14 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
15 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
16 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
17 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
18 E3.0 TS4: blastocyst-stage conceptus Present Not Specified; total RNA involves: C57BL/6 * C3H Not Specified blastocyst stage.
Notes:
(a) Each lane represents 1 blastocyst. Overall, 87% of blastocysts (64/74) showed expression of Vegfa. On this gel, all samples showed expression.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
09/30/2025
MGI 6.24
The Jackson Laboratory