Gene Expression Data
Assay Details
Assay
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Reference:
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J:219773
Francis SP, et al., A short splice form of Xin-actin binding repeat containing 2 (XIRP2) lacking the Xin repeats is required for maintenance of stereocilia morphology and hearing function. J Neurosci. 2015 Feb 4;35(5):1999-2014
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7407327
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Gene symbol:
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Xirp2
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Gene name:
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xin actin-binding repeat containing 2
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Antibody:
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anti-XIRP2 (D-18)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3A E15:
embryonic day 15.0
(more )
Note: Double labeled: green - Xirp2; red - actin.
Specimen
3A E15:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
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Double labeled: green - Xirp2; red - actin.
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Specimen
3A E18:
embryonic day 18.0
(more )
Note: Double labeled: green - Xirp2; red - actin.
Specimen
3A E18:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
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Double labeled: green - Xirp2; red - actin.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
3A E18 Merge
,
3A E18 XIRP2
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Onset of expression in hair cells beginning at E18, coinciding with emergence of hair bundle.
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Specimen
3A P4:
postnatal day 4
(more )
Note: Double labeled: green - Xirp2; red - actin.
Specimen
3A P4:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
|
Formaldehyde
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Note:
|
Double labeled: green - Xirp2; red - actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
3A P4 Merge
,
3A P4 XIRP2
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Expression localized to apical region of hair cell and bundle.
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Specimen
3A P10:
postnatal day 10
(more )
Note: Double labeled: green - Xirp2; red - actin.
Specimen
3A P10:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
Formaldehyde
|
Note:
|
Double labeled: green - Xirp2; red - actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
3A P10 XIRP2
,
3A P10 Merge
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Expression localized to apical region of hair cell and bundle.
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Specimen
3A P20:
postnatal day 20
(more )
Note: Double labeled: green - Xirp2; red - actin.
Specimen
3A P20:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 20
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
Formaldehyde
|
Note:
|
Double labeled: green - Xirp2; red - actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlea |
cochlea auditory hair cell |
Present |
Regionally restricted |
3A P20 Merge
,
3A P20 XIRP2
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Expression localized to apical region of hair cell and bundle.
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Specimen
3B E18:
embryonic day 18.0
(more )
Note: Double labeled: green - Xirp2; red - actin.
Specimen
3B E18:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
Formaldehyde
|
Note:
|
Double labeled: green - Xirp2; red - actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: utricle |
vestibular hair cell |
Present |
Regionally restricted |
3B E18 Merge
,
3B E18 XIRP2
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Onset of expression at E18, specifically in apical regions of hair cells and stereocilia.
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Specimen
3B P4:
postnatal day 4
(more )
Note: Double labeled: green - Xirp2; red - actin.
Specimen
3B P4:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
Formaldehyde
|
Note:
|
Double labeled: green - Xirp2; red - actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: utricle |
vestibular hair cell |
Present |
Regionally restricted |
3B P4 XIRP2
,
3B P4 Merge
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Expression specifically in apical regions of hair cells and stereocilia.
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Specimen
3B P30:
postnatal day 30
(more )
Note: Double labeled: green - Xirp2; red - actin.
Specimen
3B P30:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
|
Double labeled: green - Xirp2; red - actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: utricle |
vestibular hair cell |
Present |
Regionally restricted |
3B P30 XIRP2
,
3B P30 Merge
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Expression is heterogeneous: strong expression in some stereocilia with weaker expression in stereocilia in same bundle.
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Specimen
3C:
postnatal day 4
(more )
Specimen
3C:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: utricle |
vestibular hair cell |
Present |
Regionally restricted |
3C XIRP2
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Expression in vestibular hair bundle.
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Specimen
8E WT:
embryonic day 18.0
(more )
Specimen
8E WT:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Specimen
9A D-18 WT utricle:
postnatal day 4
(more )
Note: Triple labeled: blue - Xirp2 (D-18 antibody); green - Xirp2 (XRP2 minus exon 6 antibody; assay MGI:7407328); red - actin.
Specimen
9A D-18 WT utricle:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
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Triple labeled: blue - Xirp2 (D-18 antibody); green - Xirp2 (XRP2 minus exon 6 antibody; assay MGI:7407328); red - actin.
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Specimen
9A D-18 WT cochlea:
postnatal day 4
(more )
Note: Triple labeled: blue - Xirp2 (D-18 antibody); green - Xirp2 (XRP2 minus exon 6 antibody; assay MGI:7407328); red - actin.
Specimen
9A D-18 WT cochlea:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
|
Note:
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Triple labeled: blue - Xirp2 (D-18 antibody); green - Xirp2 (XRP2 minus exon 6 antibody; assay MGI:7407328); red - actin.
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