Gene Expression Data
Assay Details
Assay
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Reference:
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J:149891
Qin Z, et al., ZNF536, a novel zinc finger protein specifically expressed in the brain, negatively regulates neuron differentiation by repressing retinoic acid-induced gene transcription. Mol Cell Biol. 2009 Jul;29(13):3633-43
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7398590
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Gene symbol:
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Zfp536
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Gene name:
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zinc finger protein 536
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Antibody:
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anti-ZNF536
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
3D ZNF (left):
postnatal adult
(more )
Note: Double labeled: green - Zfp536; red - Tubb3 (assay MGI:7398596).
Specimen
3D ZNF (left):
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Zfp536; red - Tubb3 (assay MGI:7398596).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
cerebral cortex neuron |
Present |
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3D Merge (left)
,
3D ZNF (left)
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Expression localized to nucleus of Tubb3-expressing neuronal cells.
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Specimen
3D ZNF (right):
postnatal adult
(more )
Note: Double labeled: green - Zfp536; red - Gfap (assay MGI:7398600).
Specimen
3D ZNF (right):
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Zfp536; red - Gfap (assay MGI:7398600).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
cerebral cortex glial cell |
Ambiguous |
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3D Merge (right)
,
3D ZNF (right)
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Expression mostly negative in Gfap-expressing glial cells.
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Specimen
3E ZNF (leftO:
postnatal adult
(more )
Note: Double labeled: green - Zfp536; red - Tubb3 (assay MGI:7398596).
Specimen
3E ZNF (leftO:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Zfp536; red - Tubb3 (assay MGI:7398596).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
hippocampal neuron |
Weak |
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3E ZNF (left)
,
3E Merge (left)
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Weak expression in Tubb3-expressing neuronal cells.
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Specimen
3E ZNF (right):
postnatal adult
(more )
Note: Double labeled: green - Zfp536; red - Gfap (assay MGI:7398600).
Specimen
3E ZNF (right):
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
|
optical section
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Embedding:
|
Paraffin
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Note:
|
Double labeled: green - Zfp536; red - Gfap (assay MGI:7398600).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
hippocampal glial cell |
Absent |
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3E Merge (right)
,
3E ZNF (right)
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No expression in Gfap-expressing glial cells.
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