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Gene Expression Data
Assay Details
Assay
Reference: J:316543 Kim JS, et al., Reciprocal localization of transcription factors YY1 and CP2c in spermatogonial stem cells and their putative roles during spermatogenesis. Acta Histochem. 2016 Sep;118(7):685-692
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7384682
Gene symbol: Yy1
Gene name: YY1 transcription factor
Antibody: Anti-YY1 (EPR4652; ab109237)
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 3A Elongation stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Strong 3A' , 3A
TS28: spermatocyte Strong 3A , 3A'
TS28: spermatid Absent 3A' , 3A
TS28: spermatogonium Absent 3A' , 3A
Specimen 3B Grouping stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3B , 3B'
TS28: spermatid Present 3B' , 3B
TS28: spermatocyte Present 3B' , 3B
TS28: spermatogonium Present 3B' , 3B
TS28: spermatozoon Absent 3B , 3B'
TS28: seminiferous tubule Present Non-Uniform 3B' , 3B Intensity of YY1 staining progressively decreased from spermatogonial stem cell through spermatocyte, spermatid, and spermatogonia.
Specimen 3C Maturation stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3C' , 3C
TS28: spermatocyte Present 3C' , 3C
TS28: spermatogonium Present 3C' , 3C
TS28: spermatozoon Absent 3C' , 3C
Specimen 3D Release stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3D , 3D'
TS28: spermatocyte Present 3D , 3D'
TS28: spermatogonium Weak 3D , 3D'
TS28: spermatid Absent 3D' , 3D
TS28: spermatozoon Absent 3D' , 3D
Specimen 3E Elongation stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Strong 3E
TS28: spermatocyte Strong 3E
TS28: spermatid Absent 3E
TS28: spermatogonium Absent 3E
Specimen 3F Grouping stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3F
TS28: spermatid Present 3F
TS28: spermatocyte Present 3F
TS28: spermatogonium Present 3F
TS28: spermatozoon Absent 3F
TS28: seminiferous tubule Present Non-Uniform 3F Intensity of YY1 staining progressively decreased from spermatogonial stem cells through spermatocyte, spermatid, and spermatogonia.
Specimen 3G Maturation stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3G
TS28: spermatocyte Present 3G
TS28: spermatogonium Present 3G
TS28: spermatozoon Absent 3G
Specimen 3H Release stage: postnatal week 12 (more )
Note: Double labeled: brown - YY1; blue - Tfcp2 (assay MGI:7384683).
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Present 3H
TS28: spermatocyte Present 3H
TS28: spermatogonium Weak 3H
TS28: spermatid Absent 3H
TS28: spermatozoon Absent 3H
Specimen Table 1 Elongation stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Moderate
TS28: spermatogonium Absent
TS28: primary spermatocyte Strong
TS28: spermatid Absent
TS28: spermatozoon Absent
Specimen Table 1 Grouping stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Strong
TS28: spermatogonium Weak
TS28: primary spermatocyte Strong
TS28: spermatid Moderate
TS28: spermatozoon Absent
Specimen Table 1 Maturation stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Moderate
TS28: spermatogonium Moderate
TS28: primary spermatocyte Strong
TS28: spermatid Weak
TS28: spermatozoon Absent
Specimen Table 1 Release stage: postnatal week 12 (more )
Structure Cell Type Level Pattern Image Note
TS28: testis male germ line stem cell Moderate
TS28: spermatogonium Weak Non-Uniform
TS28: primary spermatocyte Strong
TS28: spermatid Absent
TS28: spermatozoon Absent
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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