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Gene Expression Data
Assay Details
Assay
Reference: J:186260 Yoshida A, et al., Localization of septin proteins in the mouse cochlea. Hear Res. 2012 Jul;289(1-2):40-51
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7380400
Gene symbol: Septin4
Gene name: septin 4
Antibody: anti-SEPT4/H5C2
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3a/c: postnatal adult (more )
Note: Double labeled: green - Septin4; blue - F-actin.
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti external pillar cell of cochlea Strong 3a , 3c
TS28: organ of Corti internal pillar cell of cochlea Strong 3a , 3c
TS28: organ of Corti Deiter's cell Present Regionally restricted 3c , 3a Expression in filament-like structures lateral to outer pillar cells between basilar membrane and reticular lamina.
TS28: organ of Corti cochlea auditory hair cell Weak 3a , 3c
TS28: organ of Corti organ of Corti supporting cell Weak 3a , 3c
Specimen 3e: postnatal adult (more )
Note: Double labeled: green - Septin4; red - Tubb3 (assay MGI:7380402).
Structure Level Pattern Image Note
TS28: cochlea Present Regionally restricted 3e No overlap with Tuj1 (Tubb3) expression in nerve fibers.
Specimen 3f: postnatal adult; Septin4tm1Ksh/Septin4tm1Ksh (more )
Structure Level Pattern Image Note
TS28: cochlea Absent 3f
Specimen 3g/i: postnatal adult (more )
Note: Double labeled: green - Septin4; red - acetylated tubulin.
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti Deiter's cell Strong Regionally restricted 3i , 3g Expression overlapped with that of acetylated alpha tubulin, abundant in Deiters' cells.
Specimen 6a/d: postnatal adult (more )
Note: Double labeled: green - Septin4; red - Myo7a (assay MGI:7380409).
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti Deiter's cell Present Regionally restricted 6a , 6d Expression in filament-like structures originating from basilar membrane and extending to apices of outer hair cells, running outside of outer hair cells. Expression in phalangeal process of Deiters' cells.
Specimen 8a/c: postnatal adult; Septin5tm1Wtr/Septin5tm1Wtr (more )
Note: Double labeled: green - Septin4; red - Syp (assay MGI:7380405).
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti Deiter's cell Present Regionally restricted 8c , 8a Expression similar to that of wild type cochlea. Expression did not overlap with that of synaptophysin
Specimen E13 not shown: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: cochlea Absent No expression in cochlea through P3.
Specimen E15 not shown: embryonic day 15.0 (more )
Structure Level Pattern Image Note
TS23: cochlea Absent No expression in cochlea through P3.
Specimen P1 not shown: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: cochlea Absent No expression in cochlea through P3.
Specimen P2 not shown: postnatal day 2 (more )
Structure Level Pattern Image Note
TS27: cochlea Absent No expression in cochlea through P3.
Specimen 9a/c: postnatal day 3 (more )
Note: Double labeled: green - Septin4; red - F-actin.
Structure Cell Type Level Pattern Image Note
TS27: organ of Corti Deiter's cell Present 9c , 9a Expression first detected in outer pillar cells and Deiters' cells.
TS27: organ of Corti external pillar cell of cochlea Present 9c , 9a Expression first detected in outer pillar cells and Deiters' cells.
TS27: organ of Corti cochlear inner hair cell Weak 9c , 9a
TS27: organ of Corti cochlear outer hair cell Strong 9a , 9c
Specimen 9d/f: postnatal day 7 (more )
Note: Double labeled: green - Septin4; red - F-actin.
Structure Cell Type Level Pattern Image Note
TS28: organ of Corti Deiter's cell Present 9d , 9f Expression first detected in outer pillar cells and Deiters' cells.
TS28: organ of Corti external pillar cell of cochlea Present 9d , 9f Expression first detected in outer pillar cells and Deiters' cells.
TS28: organ of Corti cochlear inner hair cell Weak 9d , 9f
TS28: organ of Corti cochlear outer hair cell Strong 9f , 9d
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/07/2025
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