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Gene Expression Data
Assay Details
Assay
Reference: J:236404 Minocha S, et al., Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification. Dev Biol. 2016 Oct 1;418(1):75-88
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7356406
Gene symbol: Hnf4a
Gene name: hepatic nuclear factor 4, alpha
Antibody: anti-HNF-4 alpha/NR2A1 (PP-H1415-00)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 8A: embryonic day 6.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present 8A3/4 , 8A2
TS9: embryonic visceral endoderm Present 8A3/4 , 8A2 Lower expression compared with the extraembryonic visceral endoderm.
Specimen 8B: embryonic day 6.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present 8B3/4 , 8B2 Similar as in the control.
TS9: embryonic visceral endoderm Present 8B2 , 8B3/4 Lower expression compared with the extraembryonic visceral endoderm.
Specimen 8C: embryonic day 7.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS11: extraembryonic visceral endoderm Present 8C3/4 , 8C2
TS11: embryonic visceral endoderm Trace Regionally restricted 8C3/4 , 8C2 Expression became restricted to the extraembryonic visceral endoderm. Only expressed in a few cells near the boundary between extraembryonic and embryonic portions of the embryo.
Specimen 8D: embryonic day 7.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS11: embryonic visceral endoderm Present 8D3/4 , 8D2 Increased ectopically throughout the visceral endoderm.
TS11: extraembryonic visceral endoderm Present 8D3/4 , 8D2 Increased ectopically throughout the visceral endoderm.
Specimen S4A: embryonic day 5.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS7: extraembryonic visceral endoderm Present S4A2/3
TS7: embryonic visceral endoderm Present S4A2/3 Lower expression compared with the extraembryonic visceral endoderm.
Specimen S4B: embryonic day 5.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS7: extraembryonic visceral endoderm Present S4B2/3 Similar as in the control.
TS7: embryonic visceral endoderm Present S4B2/3 Lower expression compared with the extraembryonic visceral endoderm.
Specimen S5A: embryonic day 8.5; Hcfc1tm1Lwh/Y (more )
Structure Level Pattern Image Note
TS13: yolk sac endoderm Present S5A2 , S5A3/4
Specimen S5B: embryonic day 8.5; Hcfc1tm1Lwh/Y, Edil3Tg(Sox2-cre)1Amc/Edil3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS13: embryo endoderm Present Regionally restricted S5B2 , S5B3/4 Persistent presence of ectopic expression in the embryonic visceral endoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory