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Gene Expression Data
Assay Details
Assay
Reference: J:226322 Roux M, et al., Hoxb1 regulates proliferation and differentiation of second heart field progenitors in pharyngeal mesoderm and genetically interacts with Hoxa1 during cardiac outflow tract development. Dev Biol. 2015 Oct 15;406(2):247-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7345836
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: anti-Islet1
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2J: embryonic day 9.5 (more )
Note: Double labeled: Red - Isl1; green - anti-pH3 antibody.
Structure Level Pattern Image Note
TS15: branchial arch endoderm Present 2J Pharyngeal endoderm.
TS15: secondary heart field Present 2J
Specimen 2K: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Note: Double labeled: Red - Isl1; green - anti-pH3 antibody.
Structure Level Pattern Image Note
TS15: branchial arch endoderm Present 2K Similar expression in the pharyngeal endoderm as in the wild-type.
TS15: secondary heart field Present 2K Similar as in the wild-type.
Specimen S1A/C: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Note: Sagittal sections. Double labeled: red - Isl1; green - Hoxb1 (assay MGI:7345840).
Structure Level Pattern Image Note
TS15: secondary heart field Present S1A , S1C Coexpressed with Hoxb1.
TS15: branchial arch endoderm Present S1C , S1A Coexpressed with Hoxb1 in pharyngeal endoderm.
Specimen S1D/F: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1+ (more )
Note: Transverse sections. Double labeled: red - Isl1; green - Hoxb1 (assay MGI:7345840).
Structure Level Pattern Image Note
TS15: secondary heart field Present S1F , S1D Coexpressed with Hoxb1.
TS15: branchial arch endoderm Present S1F , S1D Coexpressed with Hoxb1 in pharyngeal endoderm.
Specimen S3A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: secondary heart field Present S3A
TS15: outflow tract Present Regionally restricted S3A Expressed in the distal region.
Specimen S3B: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Structure Level Pattern Image Note
TS15: secondary heart field Present S3B Similar as in the wild-type.
TS15: outflow tract Present Regionally restricted S3B Expressed in the distal region, similar as in the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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