Pecam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:327630 Li R, et al., Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis. 2022 Jul;60(6-7):e23488
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7343700
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:	anti-CD31 (D8V9E; 77699)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Staining was visualized using tyramide-conjugated fluorophores (NEON 4-color Allround Discovery Kit for FFPE, Histova Biotechnology, NEFP450).

Results

S3a CD31 ©

S3a Merge ©

Specimen S3a: postnatal day 21; Gt(ROSA)26Sor^{tm10(CAG-Confetti2)Bzsh}/Gt(ROSA)26Sor⁺, Ihh^{em1(Tomm20/mKate2,dre)Xya}/Ihh⁺ (more

)
Note: Conditional mutant. Double labeled: red - Pecam1; cyan - Dre reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: choroidal vasculature	blood vessel endothelial cell	Present		S3a CD31 , S3a Merge , S3a Merge inset

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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