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Gene Expression Data
Assay Details
Assay
Reference: J:242917 Meng Y, et al., Pten facilitates epiblast epithelial polarization and proamniotic lumen formation in early mouse embryos. Dev Dyn. 2017 Jul;246(7):517-530
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7343561
Gene symbol: Tjp1
Gene name: tight junction protein 1
Antibody: anti-ZO-1 (# 61-7300)
Detection system: Alexa Fluor
Results
Specimen 3B Long: embryonic day 5.5 (more )
Note: Double labeled: red - Tjp1; green - Cdh1 (assay MGI:7343558). The genetic background of this specimen was either wild-type or heterozygous.
Structure Level Pattern Image Note
TS8: embryo Present Regionally restricted 3B Long. sec. ZO1 bottom , 3B Long. sec. Merge top , 3B Long. sec. ZO1 top , 3B Long. sec. Merge bottom Expression is in several rosettes with an emerging focal site.
Specimen 3B Cross: embryonic day 5.5 (more )
Note: Double labeled: red - Tjp1; green - Cdh1 (assay MGI:7343558). The genetic background of this specimen was either wild-type or heterozygous.
Structure Level Pattern Image Note
TS8: embryo Present Regionally restricted 3B Cross sec. ZO1 bottom , 3B Cross sec. ZO1 top , 3B Cross sec. Merge bottom , 3B Cross sec. Merge top Expression is in several rosettes with an emerging focal site.
Specimen 3C Long.: embryonic day 5.5; Edil3Tg(Sox2-cre)1Amc/Edil3+, Ptentm1.1Hwu/Ptentm1.1Hwu (more )
Note: Conditional mutant. Double labeled: red - Tjp1; green - Cdh1 (assay MGI:7343558).
Structure Level Pattern Image Note
TS8: embryo Present 3C Long. sec. Merge top , 3C Long. sec. ZO1 bottom , 3C Long. sec. Merge bottom , 3C Long. sec. ZO1 top In this mutant expression was scattered and marked a few rosettes and no dominant focus was present.
Specimen 3C Cross: embryonic day 5.5; Edil3Tg(Sox2-cre)1Amc/Edil3+, Ptentm1.1Hwu/Ptentm1.1Hwu (more )
Note: Conditional mutant. Double labeled: red - Tjp1; green - Cdh1 (assay MGI:7343558).
Structure Level Pattern Image Note
TS8: embryo Present Regionally restricted 3C Cross sec. Merge bottom , 3C Cross sec. ZO1 bottom , 3C Cross sec. ZO1 top , 3C Cross sec. Merge top In this mutant expression was scattered and marked a few rosettes and no dominant focus was present.
Specimen 4A WT: embryonic day 6.5 (more )
Note: Double labeled: red - Tjp1; green - Cdh1 (assay MGI:7343558). The genetic background of this specimen was either wild-type or heterozygous.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 4A WT Merge , 4A WT ZO1 Expression is in tight junctions lining the epiblast around the embryonic cavity.
Specimen 4A -: embryonic day 6.5; Edil3Tg(Sox2-cre)1Amc/Edil3+, Ptentm1.1Hwu/Ptentm1.1Hwu (more )
Note: Conditional mutant. Double labeled: red - Tjp1; green - Cdh1 (assay MGI:7343558).
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 4A - ZO1 , 4A - Merge This mutant lacks asymmetric distribution of expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory