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Gene Expression Data
Assay Details
Assay
Reference: J:327630 Li R, et al., Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis. 2022 Jul;60(6-7):e23488
Assay type: Recombinase reporter
MGI Accession ID: MGI:7341723
Gene symbol: Ihh
Gene name: Indian hedgehog
Reporter: CFP
Antibody: anti-GFP (2956)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Staining was visualized using tyramide-conjugated fluorophores (NEON 4-color Allround Discovery Kit for FFPE, Histova Biotechnology, NEFP450).
Results
Specimen Fig2(c) P1: postnatal day 1; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: femur hypertrophic chondrocyte Present 2c left in both pre-hypertrophic and hypertrophic chondrocites
TS27: bone marrow Present Single cells 2c left
Specimen Fig2(c) P12: postnatal day 12; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: femur hypertrophic chondrocyte Present 2c right in both pre-hypertrophic and hypertrophic chondrocites
TS28: bone marrow Present Single cells 2c right
Specimen Fig3(b): postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: ovary preantral follicle granulosa cell Present 3b
TS28: ovary preantral follicle theca cell Present 3b
TS28: oocyte of preantral follicle Present 3b
Specimen Fig3(e): postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: ovary antral follicle granulosa cell Present 3e
TS28: ovary antral follicle theca cell Present 3e
TS28: oocyte of antral follicle Present 3e
Specimen Fig3(h): postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: corpus luteum luteinizing hormone secreting cell Present 3h
TS28: corpus luteum mural cell Present 3h
Specimen Fig3(k): postnatal day 120; Gt(ROSA)26Sortm10(CAG-Confetti2)Bzsh/Gt(ROSA)26Sor+, Ihhem1(Tomm20/mKate2,dre)Xya/Ihh+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS28: corpus luteum luteinizing hormone secreting cell Present 3k
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
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