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Gene Expression Data
Assay Details
Assay
Reference: J:327232 Wu JPH, et al., The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the Mouse. Front Mol Neurosci. 2022;15:921901
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7336061
Gene symbol: Pou3f1
Gene name: POU domain, class 3, transcription factor 1
Antibody: anti-OCT6 (clone KT110) (MABN738)
Detection system: Secondary antibody
Results
Specimen 2B: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS18: cerebellum primordium Present Regionally restricted 2B Expression in cells along subpial stream.

Specimen 2C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebellum primordium Present Regionally restricted 2C Between E12.5 and E15.5 expression in cells in nuclear transitory zone and nowhere else in the developing cerebellum.

Specimen 2F: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: cerebellum white matter Present Regionally restricted 2F Expression in clusters of cells in cerebellar white matter in the forming nuclear region.

Specimen 2G: postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - vGluT2 (Slc17a6 assay MGI:7336062).
Structure Level Pattern Image Note
TS28: cerebellum white matter Present Regionally restricted 2G (left) , 2G (right) Nuclear expression in cells co-expressing vGlut2 (Slc17a6).

Specimen 3A: postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - GAD67 (Gad1 assay MGI:7336063).
Structure Level Pattern Image Note
TS28: brain Present Regionally restricted 3A No co-expression with GAD67 (Gad1).
TS28: cerebellum white matter Present Regionally restricted 3D , 3B , 3A Expression in cells not surrounded by GAD67 (Gad1)-expressing puncta.

Specimen 4A: embryonic day 12.5 (more )
Note: Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
Structure Level Pattern Image Note
TS20: cerebellum primordium Present Regionally restricted 4A
TS20: rhombic lip Absent 4A

Specimen 4B: embryonic day 12.5 (more )
Note: Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
Structure Level Pattern Image Note
TS20: rhombic lip Absent 4B No expression in posterior and lateral part of rhombic lip.

Specimen 4C: embryonic day 12.5 (more )
Note: Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
Structure Level Pattern Image Note
TS20: cerebellum primordium Present Regionally restricted 4C Expression co-localized with Atoh1 in part of subpial stream proximal to rhombic lip.

Specimen 4D: embryonic day 12.5 (more )
Note: Double labeled: red - Pou3f1; green - Atoh1 (assay MGI:7336064).
Structure Level Pattern Image Note
TS20: cerebellum primordium Present Regionally restricted 4D Expression in more distal parts of subpial stream.

Specimen 5A: embryonic day 13.5 (more )
Note: Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
Structure Level Pattern Image Note
TS21: cerebellum Present Regionally restricted 5A Expression predomininantly in nuclear transitory zone; not co-localized with Pax6 expression in rhombic lip and beginning of subpial stream.
TS21: rhombic lip Absent 5A

Specimen 5A": embryonic day 13.5 (more )
Note: Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
Structure Level Pattern Image Note
TS21: cerebellum Present Regionally restricted 5A' Expression in cells localized to the nuclear transitory zone.

Specimen 5A'': embryonic day 13.5 (more )
Note: Double labeled: red - Pou3f1; green - Pax6 (assay MGI:7336065).
Structure Level Pattern Image Note
TS21: rhombic lip Absent 5A'' Expression not co-localized with Pax6 in rhombic lip and beginning of subpial stream.

Specimen 6A: embryonic day 13.5 (more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Structure Level Pattern Image Note
TS21: cerebellum Present Regionally restricted 6A Expression in cells located more medially than Tbr1-expressing cells.

Specimen 6B: embryonic day 15.5 (more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Structure Cell Type Level Pattern Image Note
TS23: cerebellum cerebellar neuron Present Regionally restricted 6B Expression in cerebellar nuclear (CN) neurons in same plate along medial-lateral axis and Tbr1-expressing CN neurons, but Pou3f1-expressing CN neurons tend to cluster more ventrally.

Specimen 6C: postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum cerebellar neuron Present Regionally restricted 6C Expression in cluster of in cerebellar nuclear (CN) neurons more lateral than cluster of Tbr1-expressing CN neurons.

Specimen 6D,D': postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Structure Level Pattern Image Note
TS28: cerebellum interpositus nucleus Present 6D , 6D' Expression primarily in interposed and dentate nuclei.

Specimen 6E,E': postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Structure Level Pattern Image Note
TS28: fastigial nucleus Absent 6E' , 6E

Specimen 6F,F': postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Tbr1 (assay MGI:7336066).
Structure Level Pattern Image Note
TS28: dentate nucleus Present 6F , 6F' Expression primarily in interposed and dentate nuclei.

Specimen 7B: postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 7B Co-expression with Brn2 (Pou3f2). Some Pou3f1-expressing cells that are not labele by Brn2.

Specimen 7C,C': postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
Structure Level Pattern Image Note
TS28: cerebellum interpositus nucleus Present 7C' , 7C Co-expression with (Pou3f2) in interposed nucleus. Cohort of Brn2+/Pou3f1- cells.

Specimen 7D,D': postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Brn2 (Pou3f2 assay MGI:7336068).
Structure Level Pattern Image Note
TS28: dentate nucleus Present 7D , 7D' Co-expression with (Pou3f2) in dentate nucleus. Cohort of Brn2+/Pou3f1- cells.

Specimen 9A: postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
Structure Level Pattern Image Note
TS28: cerebellum Present Regionally restricted 9A Co-expression with Irx3.

Specimen 9B,B': postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
Structure Level Pattern Image Note
TS28: cerebellum interpositus nucleus Present 9B' , 9B Co-expression with Irx3 predominantly in interposed nucleus.

Specimen 9C,C': postnatal day 6 (more )
Note: Double labeled: red - Pou3f1; green - Irx3 (assay MGI:7336070).
Structure Level Pattern Image Note
TS28: dentate nucleus Present 9C' , 9C Minor co-expression with Irx3 in dentate nucleus.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory