Gene Expression Data
Assay Details
Assay
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Reference:
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J:327232
Wu JPH, et al., The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the Mouse. Front Mol Neurosci. 2022;15:921901
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7336060
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Gene symbol:
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Pou3f1
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Gene name:
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POU domain, class 3, transcription factor 1
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Results
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Specimen
2D:
embryonic day 15.5
(more )
Specimen
2D:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS23: cerebellum |
Present |
Regionally restricted |
2D
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Between E12.5 and E15.5 expression in cells in nuclear transitory zone and nowhere else in developing cerebellum.
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Specimen
2E:
embryonic day 18.5
(more )
Specimen
2E:
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebellum white matter |
Present |
Regionally restricted |
2E
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Expression in clusters of cells in cerebellar white matter in the forming nuclear region.
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Specimen
4E,E':
embryonic day 15.5
(more )
Specimen
4E,E':
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: cerebellum |
cerebellum glutamatergic neuron |
Present |
Regionally restricted |
4E'
,
4E
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Expression in nuclear transitory zone. Expression in glutamatergic cerebellar nuclear (CN) neurons.
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Specimen
4F,F':
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Atoh1tm2Hzo/Atoh1tm2Hzo
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS23: cerebellum |
Trace |
Single cells |
4F
,
4F'
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Expression completely absent in nuclear transitory zone. Residual Pou3f1 expression attributable to very few residual Atoh1-expressing cells.
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Specimen
5B,B':
embryonic day 18.5
(more )
Specimen
5B,B':
(close )
Genetic Background:
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C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5C,C':
(close )
Genetic Background:
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involves: 102 * C3H
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Mutant Allele(s):
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Pax6Sey-Neu/Pax6Sey-Neu
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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