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Gene Expression Data
Assay Details
Assay
Reference: J:232723 Qu C, et al., Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells. J Biol Chem. 2016 Apr 1;291(14):7661-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7334943
Gene symbol: Pou4f2
Gene name: POU domain, class 4, transcription factor 2
Antibody: anti-Brn3b (N-15) (sc-31989)
Detection system: Secondary antibody
Results
Specimen 2B: embryonic day 16.5 (more )
Note: Double labeled: red - Brn3b (Pou4f2); green - Fezf2 (assay MGI:7334942).
Structure Level Pattern Image Note
TS24: retina outer nuclear layer Present Regionally restricted 2B BRN3B , 2B MERGE Expression co-localized with Fezf2.
Specimen 3E BRN3B wild-type (upper): embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: retina Present 3E BRN3B wild-type (upper)
Specimen 3E BRN3B Fezf2-/- (upper): embryonic day 17.5; Fezf2tm1.1Nses/Fezf2tm1.1Nses, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: retina Weak 3E BRN3B Fezf2-/- (upper) Reduction in expressing cells compared to wild-type.
Specimen 3E BRN3B wild-type (lower): embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: retina Present Regionally restricted 3E BRN3B wild-type (lower)
TS25: retina nuclear layer Present Scattered 3E BRN3B wild-type (lower) Few expressing cells scattered in neuroblastic layer.
Specimen 3E BRN3B Fezf2-/- (lower): embryonic day 17.5; Fezf2tm1.1Nses/Fezf2tm1.1Nses, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: retina Weak 3E BRN3B Fezf2-/- (lower) Reduction in expressing cells compared to wild-type.
TS25: retina nuclear layer Trace 3E BRN3B Fezf2-/- (lower) Almost complete reduction of expressing cells in neuroblastic layer.
Specimen 4A wild-type: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: retina Present 4A wild-type
Specimen 4A Fezf2-/-: postnatal day 7; Fezf2tm1.1Nses/Fezf2tm1.1Nses, Tg(Six3-cre)69Frty/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: retina Weak 4A Fezf2-/- Number of expressing cells significantly reduced to ~47.3% compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory