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Gene Expression Data
Assay Details
Assay
Reference: J:307271 Airik M, et al., Loss of Anks6 leads to YAP deficiency and liver abnormalities. Hum Mol Genet. 2020 Nov 4;29(18):3064-3080
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7333214
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen S5a,a': embryonic day 15.5 (more )
Note: Double labeled: red - alpha-SMA (Acta2); green - Sox9 (assay MGI:7333215).
Structure Cell Type Level Pattern Image Note
TS23: liver myofibroblast cell Present S5a , S5a'
Specimen S5b,b': embryonic day 15.5; Anks6tm1d(KOMP)Wtsi/Anks6tm1d(KOMP)Wtsi (more )
Note: Double labeled: red - alpha-SMA (Acta2); green - Sox9 (assay MGI:7333215).
Structure Cell Type Level Pattern Image Note
TS23: liver myofibroblast cell Present S5b' , S5b No significant change compared to wild type.
Specimen 3A: embryonic day 18.5 (more )
Note: Double labeled: red - alpha-SMA (Acta2); green - Alb (assay MGI:7333218).
Structure Cell Type Level Pattern Image Note
TS26: liver pericyte Present Regionally restricted 3A Expression in layer of perivascular cells (pericyte cell layer) underneath portal endothelium.
Specimen 3B: embryonic day 18.5; Anks6tm1d(KOMP)Wtsi/Anks6tm1d(KOMP)Wtsi (more )
Note: Double labeled: red - alpha-SMA (Acta2); green - Alb (assay MGI:7333218).
Structure Cell Type Level Pattern Image Note
TS26: liver Present Regionally restricted 3B
TS26: liver parenchyma myofibroblast cell Present Regionally restricted 3B Expression in myofibroblasts extending into liver parenchyma between hepatocytes.
Specimen 3C: embryonic day 18.5 (more )
Note: Double labeled: red - alpha-SMA (Acta2); green - pan-cytokeratin.
Structure Cell Type Level Pattern Image Note
TS26: liver pericyte Present Regionally restricted 3C Expression in layer of perivascular cells underneath portal endothelium.
Specimen 3D: embryonic day 18.5; Anks6tm1d(KOMP)Wtsi/Anks6tm1d(KOMP)Wtsi (more )
Note: Double labeled: red - alpha-SMA (Acta2); green - pan-cytokeratin.
Structure Cell Type Level Pattern Image Note
TS26: liver Present Regionally restricted 3D Expression extends throughout periportal mesenchyme.
TS26: liver parenchyma myofibroblast cell Present Regionally restricted 3D Expression in myofibroblasts surrounding developing bile ducts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory