Gene Expression Data
Assay Details
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Assay
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Reference:
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J:238836
Adams DC, et al., Follistatin-like 1 regulates renal IL-1beta expression in cisplatin nephrotoxicity. Am J Physiol Renal Physiol. 2010 Dec;299(6):F1320-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7327057
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Gene symbol:
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Fstl1
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Gene name:
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follistatin-like 1
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Antibody:
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Anti-FSTL1 (AF1738)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 568
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Results
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Specimen
3B:
embryonic day 17.5
(more  )
Note: Double labeled: red - Fstl1; green - dolichos bifloris agglutinin (DBA).
Specimen
3B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Fstl1; green - dolichos bifloris agglutinin (DBA).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: renal tubule |
Present |
Regionally restricted |
3C
,
3B
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Immunopositive tubule segments in the embryonic kidney are distal, as they can be seen to fuse with collecting ducts within the nephrogenic zone.
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| TS25: medullary collecting duct |
Present |
Regionally restricted |
3B
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Limited protein expression was noted in collecting ducts located in the medullary region of the kidney, which had been co-labeled with the collecting duct marker dolichos bifloris agglutinin (DBA).
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Specimen
3F:
postnatal adult
(more )
Note: Double labeled: red - Fstl1; green - lotus tetragonolobus
lectin (LTL).
Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Fstl1; green - lotus tetragonolobus
lectin (LTL).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: loop of Henle, outer medullary portion |
Present |
Regionally restricted |
3F
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Expression in the adult organ was detected in a sparse population of thin tubules in the outer medulla that does not colocalize with LTL. This expression was thought to be a segment of the loop of Henle.
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| TS28: renal proximal tubule |
Absent |
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3F
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The immunopositive tubules do not colocalize with the proximal tubule marker lotus tetragonolobus lectin (LTL).
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Specimen
not shown:
postnatal adult
(more )
Note: Double labeled: red - Fstl1; green - dolichos bifloris agglutinin (DBA).
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Fstl1; green - dolichos bifloris agglutinin (DBA).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: collecting duct |
Absent |
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The immunopositive tubules do not colocalize with collecting duct marker dolichos bifloris agglutinin (DBA).
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