Nanog RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:326575 Tsume-Kajioka M, et al., BET proteins are essential for the specification and maintenance of the epiblast lineage in mouse preimplantation embryos. BMC Biol. 2022 Mar 9;20(1):64
Assay type:	RNA in situ
MGI Accession ID:	MGI:7316609
Gene symbol:	Nanog
Gene name:	Nanog homeobox

Probe:	Nanog probe5
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

6E ©

Specimen 6E: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present		6E	Expression in epiblast cells.

6J ©

Specimen 6J: embryonic day 3.5; Brd4^{tm1a(EUCOMM)Wtsi}/Brd4^{tm1a(EUCOMM)Wtsi}, Tmem163^{Tg(ACTB-cre)2Mrt}/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Weak		6J	Expression tended to be reduced but not diminished.

S9F ©

Specimen S9F: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present		S9F

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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