Nanog Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:326575 Tsume-Kajioka M, et al., BET proteins are essential for the specification and maintenance of the epiblast lineage in mouse preimplantation embryos. BMC Biol. 2022 Mar 9;20(1):64
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7316599
Gene symbol:	Nanog
Gene name:	Nanog homeobox

Antibody:	anti-Nanog (RCAB0002P-F/ RCAB001P)
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Antibody name MGI:5882064 may have been used in this assay.

Results

3A ©

Specimen 3A: embryonic day 3.0 (more

)
Note: 16 cell stage

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present	Widespread	3A	Expression in most blastomeres at !6-cell morula stage

S1C ©

S1C' ©

Specimen S1C,C': embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	S1C' , S1C	Co-expression with Gata6 in some cells; mostly mutually exclusive expression.

5B ©

5C ©

5C' ©

Specimen 5B,C,C': embryonic day 3.5 (more

)
Note: Double labeled: magenta - Nanog; green - phophorylated Stat3 (assay MGI:7316606).

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Present	Regionally restricted	5C , 5B , 5C'	Expression in cells that express pSTAT3; not in nuclei.

6B ©

Specimen 6B: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present		6B

6C ©

Specimen 6C: embryonic day 3.5; Brd4^{tm1a(EUCOMM)Wtsi}/Brd4^{tm1a(EUCOMM)Wtsi}, Tmem163^{Tg(ACTB-cre)2Mrt}/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present		6C	Expression not reduced compared with wild type.

S8A ©

S8C ©

S8C' ©

Specimen S8A,C,C': embryonic day 4.25 (more

)
Note: Triple labeled: magenta - Nanog; green - Gata6 (assay MGI:7316604); yellow - Cdx2 (assay MGI:7316605).

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		S8A , S8C' , S8C

S8B ©

S8D ©

S8D' ©

Specimen S8B,D,D': embryonic day 4.25; Brd4^{tm1a(EUCOMM)Wtsi}/Brd4^{tm1a(EUCOMM)Wtsi}, Tmem163^{Tg(ACTB-cre)2Mrt}/0 (more

)
Note: Conditional mutant. Triple labeled: magenta - Nanog; green - Gata6 (assay MGI:7316604); yellow - Cdx2 (assay MGI:7316605).

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present	Regionally restricted	S8D' , S8D , S8B	Number of expressing cells decreased compared to wild type.

S9H ©

Specimen S9H: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS5: blastocyst	Present		S9H

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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