Gcna Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:133516 Alton M, et al., The behavior of the X- and Y-chromosomes in the oocyte during meiotic prophase in the B6.Y(TIR)sex-reversed mouse ovary. Reproduction. 2008 Feb;135(2):241-52
Assay type:	Western blot
MGI Accession ID:	MGI:7314917
Gene symbol:	Gcna
Gene name:	germ cell nuclear acidic protein

Antibody:	anti-GCNA (10D9G11)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Image: 9GCNA

	Sample Information		Bands					Other Sample Information
Lane	Age	Structure	95 kDa (a)	81 kDa (b)	79 kDa (b)	67 kDa (b)	65 kDa (b)	Genetic Background	Mutant Allele(s)	Sex	Note
14.5 XX	E14.5	TS22: ovary	Present	Present	Present	Present	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1		Female
14.5 XY	E14.5	TS22: ovary	Present	Present	Present	Present	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed
17.5 XX	E17.5	TS25: ovary	Present	Present	Present	Present	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1		Female
17.5 XY	E17.5	TS25: ovary	Present	Present	Present	Present	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed
20.5 dpc XX	P newborn	TS27: ovary	Present	Present	Present	Present	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1		Female
20.5 dpc XY	P newborn	TS27: ovary	Weak	Present	Present	Present	Present	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed

Notes:
(a) The band intensities were comparable in the XX and XY ovaries at 14.5 and 17.5 dpc. The intensity of the 95kDa band was consistently lower (70%) than in the XX ovary at 20.5dpc.
(b) The band intensities were comparable in the XX and XY ovaries.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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