Uba1y RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:133516 Alton M, et al., The behavior of the X- and Y-chromosomes in the oocyte during meiotic prophase in the B6.Y(TIR)sex-reversed mouse ovary. Reproduction. 2008 Feb;135(2):241-52
Assay type:	RT-PCR
MGI Accession ID:	MGI:7314866
Gene symbol:	Uba1y
Gene name:	ubiquitin-activating enzyme, Chr Y

Probe:	Ube1y-pA, Ube1y-pB
Visualized with:	Ethidium bromide
Assay notes:	Semi-quantitative RT-PCR. Relative transcript levels against beta-actin.

Results

Image: 8A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	330 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
B6.Y^TIROv E11.5	E11.5	TS19: gonad	Ambiguous	Not Specified; total RNA	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed
B6.Y^TIROv E12.5	E12.5	TS20: ovary	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed
B6.Y^TIROv E13.5	E13.5	TS21: ovary	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed
B6.Y^TIROv E17.5	E17.5	TS25: ovary	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	sex reversed
B6.Y^TIROT E11.5	E11.5	TS19: gonad	Ambiguous	Not Specified; total RNA	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	ovotestes
B6.Y^TIROT E12.5	E12.5	TS20: gonad	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	ovotestes
B6.Y^TIROT E13.5	E13.5	TS21: gonad	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	ovotestes
B6.Y^TIROT E17.5	E17.5	TS25: gonad	Present	Not Specified; total RNA	(C57BL/6J x C57BL/6-Chr Y^Tirano/Na)F1	X/Sry^POS-TIR	Not Specified	ovotestes
B6.XY E11.5	E11.5	TS19: gonad	Ambiguous	Not Specified; total RNA	C57BL/6		Male
B6.XY E12.5	E12.5	TS20: testis	Present	Not Specified; total RNA	C57BL/6		Male
B6.XY E13.5	E13.5	TS21: testis	Present	Not Specified; total RNA	C57BL/6		Male
B6.XY E17.5	E17.5	TS25: testis	Present	Not Specified; total RNA	C57BL/6		Male

Notes:
(a) Ube1y transcript levels increased from E11.5 to E13.5 regardless of the gonadal phenotype. Levels remained high in B.Y<TIR> ovaries but declined in both B6.Y<TIR> ovotestes and B6.XY testes.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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