Sox9 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:324823 Mitchel K, et al., Hoxa5 Activity Across the Lateral Somitic Frontier Regulates Development of the Mouse Sternum. Front Cell Dev Biol. 2022;10:806545
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7309996
Gene symbol:	Sox9
Gene name:	SRY (sex determining region Y)-box 9

Antibody:	Anti-Sox9 (AB5535)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

S3A ©

S3A" ©

S3G ©

Specimen S3A/G: embryonic day 14.5 (more

)
Note: Interclavicular mesenchyme. Double labeled: red - Sox9; green - Hoxa5 (assay MGI:7309976).

Structure	Level	Pattern	Image	Note
TS22: clavicle cartilage condensation	Present		S3A" , S3A , S3G
TS22: sternum	Present		S3G , S3A , S3A"	At this anterior-most point, the presternum contained: paired dorsal elements that contact the clavicles; separate paired elements that lie ventral to the clavicle, and a ventral-midline element where pectoral muscle attachment occurs.

S3B ©

S3B" ©

S3H ©

Specimen S3B/H: embryonic day 14.5 (more

)
Note: Interclavicular mesenchyme. Double labeled: red - Sox9; green - Hoxa5 (assay MGI:7309976).

Structure	Level	Pattern	Image	Note
TS22: sternum	Present		S3H , S3B , S3B"	At this anterior-most point, the presternum contained: paired dorsal elements that contact the clavicles; separate paired elements that lie ventral to the clavicle, and a ventral-midline element where pectoral muscle attachment occurs.

S3C ©

S3C" ©

S3I ©

Specimen S3C/I: embryonic day 14.5 (more

)
Note: Intercostoclavicular mesenchyme. Double labeled: red - Sox9; green - Hoxa5 (assay MGI:7309976).

Structure	Level	Pattern	Image	Note
TS22: sternum	Present		S3C , S3I , S3C"	At the axial level of the intercostoclavicular, the ventral paired elements are larger and bar-shaped with increased expression.

S3D ©

S3D" ©

S3J ©

Specimen S3D/J: embryonic day 14.5 (more

)
Note: Intercostoclavicular mesenchyme. Double labeled: red - Sox9; green - Hoxa5 (assay MGI:7309976).

Structure	Level	Pattern	Image	Note
TS22: sternum	Present		S3J , S3D , S3D"	At the axial level of the intercostoclavicular, the ventral paired elements are larger and bar-shaped with increased expression.

S3E ©

S3E" ©

S3K ©

Specimen S3E/K: embryonic day 14.5 (more

)
Note: Rib 1. Double labeled: red - Sox9; green - Hoxa5 (assay MGI:7309976).

Structure	Level	Pattern	Image	Note
TS22: T1 rib cartilage condensation	Present		S3E" , S3K , S3E
TS22: sternum	Present		S3E" , S3K , S3E

S3F ©

S3F" ©

S3L ©

Specimen S3F/L: embryonic day 14.5 (more

)
Note: Rib 2. Double labeled: red - Sox9; green - Hoxa5 (assay MGI:7309976).

Structure	Level	Pattern	Image	Note
TS22: sternum	Present		S3F" , S3L , S3F

S5A ©

Specimen S5A: embryonic day 14.5 (more

)
Note: Intercostoclavicular mesenchyme. Double labeled: red - Sox9; green - Casp3 (assay MGI:7309999).

Structure	Level	Pattern	Image	Note
TS22: sternum	Present		S5A

S5E ©

Specimen S5E: embryonic day 14.5; Hoxa5^tm1Rob/Hoxa5^tm1Rob (more

)
Note: Intercostoclavicular mesenchyme. Double labeled: red - Sox9; green - Casp3 (assay MGI:7309999).

Structure	Level	Pattern	Image	Note
TS22: sternum	Present		S5E

S5C ©

Specimen S5C: embryonic day 14.5 (more

)
Note: Rib 1. Double labeled: red - Sox9; green - Casp3 (assay MGI:7309999).

Structure	Level	Pattern	Image	Note
TS22: T1 rib cartilage condensation	Present		S5C
TS22: sternum	Present		S5C

S5G ©

Specimen S5G: embryonic day 14.5; Hoxa5^tm1Rob/Hoxa5^tm1Rob (more

)
Note: Rib 1. Double labeled: red - Sox9; green - Casp3 (assay MGI:7309999).

Structure	Level	Pattern	Image	Note
TS22: T1 rib cartilage condensation	Present		S5G
TS22: sternum	Present		S5G

S5I ©

Specimen S5I: embryonic day 14.5 (more

)
Note: Intercostoclavicular mesenchyme. Double labeled: red - Sox9; green - Pcna (assay MGI:7310000).

Structure	Level	Pattern	Image	Note
TS22: sternum	Present		S5I

S5M ©

Specimen S5M: embryonic day 14.5; Hoxa5^tm1Rob/Hoxa5^tm1Rob (more

)
Note: Intercostoclavicular mesenchyme. Double labeled: red - Sox9; green - Pcna (assay MGI:7310000).

Structure	Level	Pattern	Image	Note
TS22: sternum	Present		S5M

S5K ©

Specimen S5K: embryonic day 14.5 (more

)
Note: Rib 1. Double labeled: red - Sox9; green - Pcna (assay MGI:7310000).

Structure	Level	Pattern	Image	Note
TS22: T1 rib cartilage condensation	Present		S5K
TS22: sternum	Present		S5K

S5O ©

Specimen S5O: embryonic day 14.5; Hoxa5^tm1Rob/Hoxa5^tm1Rob (more

)
Note: Rib 1. Double labeled: red - Sox9; green - Pcna (assay MGI:7310000).

Structure	Level	Pattern	Image	Note
TS22: T1 rib cartilage condensation	Present		S5O
TS22: sternum	Present		S5O

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