Antibody:	anti-Dlk1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Absent		2e

Structure	Level	Pattern	Image	Note
TS24: adenohypophysis pars anterior	Present		2f
TS24: adenohypophysis pars intermedia	Present		2f
TS24: hypothalamus	Present		2f
TS24: optic chiasma	Present		2f
TS24: remnant of Rathke's pouch	Present		2f

Structure	Level	Pattern	Image	Note
TS24: brain subventricular zone	Present		2g	Expression in ependymal epithelium and subependymal areas of ventricles.
TS24: brain ventricular layer	Present		2g	Expression in ependymal epithelium and subependymal areas of ventricles.
TS24: choroid plexus epithelium	Present		2g

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Absent		2h

Structure	Level	Pattern	Image	Note
TS24: intervertebral disc	Strong	Regionally restricted	3d	Strongest expression in cartilage of intervertebral bodies.
TS24: vertebra	Trace		3d	Low expression in vertebral bodies.

Structure	Level	Pattern	Image	Note
TS24: nucleus pulposus	Weak		3e

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Meckel's cartilage	chondrocyte	Present	Regionally restricted	4d	Expression in chondrocyte cell membranes and cytoplasm

Structure	Cell Type	Level	Pattern	Image	Note
TS24: digit mesenchyme	chondrocyte	Present	Regionally restricted	4e	Expression in chondrocyte cell membranes.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: rib cartilage	chondrocyte	Present	Regionally restricted	4f	Expression in chondrocyte cell membranes.

Structure	Level	Pattern	Image	Note
TS24: skeletal muscle tissue	Present	Regionally restricted	5b	Expression in fiber membrane and along myotubes.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: salivary gland mesenchyme		Present		6c
TS24: salivary gland	acinar cell	Present	Regionally restricted	6c	Expression in distal acinar cells and myoepithelial cells.
TS24: salivary gland	myoepithelial cell	Present	Regionally restricted	6c	Expression in distal acinar cells and myoepithelial cells.

Structure	Level	Pattern	Image	Note
TS24: salivary gland epithelium	Present	Regionally restricted	6d	Expression in distal acini. No expression in ducts.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: liver	hepatocyte	Present	Regionally restricted	7b	Expression in membrane of primitive hepatocytes.

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present		8c
TS24: terminal bronchiole epithelium	Present		8c
TS24: bronchiole epithelium	Present	Regionally restricted	8c	No expression in proximal bronchiolar epithelium.

Structure	Level	Pattern	Image	Note
TS24: terminal bronchiole epithelium	Present		8d

Structure	Level	Pattern	Image	Note
TS24: intestine mesenchyme	Strong	Regionally restricted	9c	Intense expression in connective tissue near basement membrane and in the muscularis layer
TS24: intestine muscularis	Strong	Regionally restricted	9c	Intense expression in connective tissue near basement membrane and in the muscularis layer

Structure	Level	Pattern	Image	Note
TS24: intestine mesenchyme	Strong	Regionally restricted	9d	Intense expression in connective tissue.
TS24: intestine	Present	Regionally restricted	9d	Low expression in submucosa

Structure	Level	Pattern	Image	Note
TS24: thymus	Present	Regionally restricted	10b	Expression in capsule and in blood vessels in cortex and medulla.

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present	Regionally restricted	11d

Structure	Level	Pattern	Image	Note
TS24: maturing glomerular tuft	Present		11e
TS24: renal convoluted tubule	Present	Regionally restricted	11e	Expression in distal convoluted tubules.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: adrenal medulla	chromaffin cell	Present		11f