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Gene Expression Data
Assay Details
Assay
Reference: J:168841 Lopes Floro K, et al., Loss of Cited2 causes congenital heart disease by perturbing left-right patterning of the body axis. Hum Mol Genet. 2011 Mar 15;20(6):1097-110
Assay type: RNA in situ
MGI Accession ID: MGI:7286315
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Probe: Pitx2 probe35
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3A: embryonic day 9.5; Mesp1tm2(cre)Ysa/Mesp1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: mesenchyme derived from lateral plate Present 3A Expression localized within pericardio-peritoneal canal and lateral mesoderm.
TS15: pericardio-peritoneal canal Present 3A Expression localized within pericardio-peritoneal canal and lateral mesoderm.
Specimen 3B: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1.1Dunw, Mesp1tm2(cre)Ysa/Mesp1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: mesenchyme derived from lateral plate Present 3B Expression localized within pericardio-peritoneal canal and lateral mesoderm.
TS15: pericardio-peritoneal canal Present 3B Expression localized within pericardio-peritoneal canal and lateral mesoderm.
Specimen 3C: embryonic day 9.5; Mesp1tm2(cre)Ysa/Mesp1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: secondary heart field Present Regionally restricted 3C Expression in left second heart field.
Specimen 3D: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1.1Dunw, Mesp1tm2(cre)Ysa/Mesp1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: secondary heart field Present Regionally restricted 3D Expression in left second heart field.
Specimen 3E: embryonic day 9.5; Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: mesenchyme derived from lateral plate Present 3E Expression localized within pericardio-peritoneal canal and lateral mesoderm.
TS15: pericardio-peritoneal canal Present 3E Expression localized within pericardio-peritoneal canal and lateral mesoderm.
Specimen 3F: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1.1Dunw, Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: mesenchyme derived from lateral plate Present 3F Expression localized within pericardio-peritoneal canal and lateral mesoderm.
TS15: pericardio-peritoneal canal Present 3F Expression localized within pericardio-peritoneal canal and lateral mesoderm.
Specimen 3G: embryonic day 9.5; Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: secondary heart field Present Regionally restricted 3G Expression in left second heart field.
Specimen 3H: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1.1Dunw, Tg(Leftb-cre)1Hmd/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: secondary heart field Present Regionally restricted 3H Expression in left second heart field.
Specimen 3I: embryonic day 10.5; Mesp1tm2(cre)Ysa/Mesp1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: heart Present Regionally restricted 3I Expression in heart including outflow tract.
TS17: outflow tract Present 3I
Specimen 3J: embryonic day 10.5; Cited2tm1Jpmb/Cited2tm1.1Dunw, Mesp1tm2(cre)Ysa/Mesp1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: heart Present Regionally restricted 3J Expression in heart including outflow tract.
TS17: outflow tract Present 3J
Specimen 5A: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: mesenchyme derived from lateral plate Present Regionally restricted 5A Expression on left side within pericardio-peritoneal canal and lateral mesoderm.
TS15: pericardio-peritoneal canal Present Regionally restricted 5A Expression on left side within pericardio-peritoneal canal and lateral mesoderm.
Specimen 5B: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1Jpmb (more )
Structure Level Pattern Image Note
TS15: mesenchyme derived from lateral plate Absent 5B Expression not detected in pericardio-peritoneal canal and lateral mesoderm.
TS15: pericardio-peritoneal canal Absent 5B Expression not detected in pericardio-peritoneal canal and lateral mesoderm.
Specimen 5C: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1Jpmb, Smad1tm1Rob/Smad1+ (more )
Structure Level Pattern Image Note
TS15: mesenchyme derived from lateral plate Present Regionally restricted 5C Expression on left side within pericardio-peritoneal canal and lateral mesoderm in some mutant embryos.
TS15: pericardio-peritoneal canal Present Regionally restricted 5C Expression on left side within pericardio-peritoneal canal and lateral mesoderm in some mutant embryos.
Specimen 5D: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1Jpmb, Smad1tm1Rob/Smad1+ (more )
Structure Level Pattern Image Note
TS15: pericardio-peritoneal canal Present Regionally restricted 5D Bilateral expression in pericardio-peritoneal canal.
TS15: mesenchyme derived from lateral plate Absent 5D Expression absent in lateral mesoderm.
Specimen 5E: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1Jpmb, Smad1tm1Rob/Smad1+ (more )
Structure Level Pattern Image Note
TS15: pericardio-peritoneal canal Present Regionally restricted 5E Expression on left side within pericardio-peritoneal canal and lateral mesoderm.
TS15: mesenchyme derived from lateral plate Absent 5E Expression absent in lateral mesoderm.
Specimen 5F: embryonic day 9.5; Cited2tm1Jpmb/Cited2+ (more )
Structure Level Pattern Image Note
TS15: mesenchyme derived from lateral plate Present Regionally restricted 5F Expression on left side within pericardio-peritoneal canal and lateral mesoderm.
TS15: pericardio-peritoneal canal Present Regionally restricted 5F Expression on left side within pericardio-peritoneal canal and lateral mesoderm.
Specimen 5G: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1Jpmb, Smad1tm1Rob/Smad1+ (more )
Structure Level Pattern Image Note
TS15: pericardio-peritoneal canal Present Regionally restricted 5G Bilateral expression around pericardio-peritoneal canal.
Specimen 5H: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1Jpmb, Smad1tm1Rob/Smad1+ (more )
Structure Level Pattern Image Note
TS15: dorsal mesocardium Strong Regionally restricted 5H Strong bilateral expression in lung bud and dorsal mesocardium.
TS15: lung Strong Regionally restricted 5H Strong bilateral expression in lung bud and dorsal mesocardium.
Specimen 5I: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1Jpmb, Smad1tm1Rob/Smad1+ (more )
Structure Level Pattern Image Note
TS15: lung Weak Regionally restricted 5I Weak left-sided lung bud expression.
Specimen 5J: embryonic day 9.5; Cited2tm1Jpmb/Cited2tm1Jpmb, Smad1tm1Rob/Smad1+ (more )
Structure Level Pattern Image Note
TS15: lung Strong Regionally restricted 5J Strong left-sided lung bud expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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