Wt1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:310902 Hayashi S, et al., The nephric mesenchyme lineage of intermediate mesoderm is derived from Tbx6-expressing derivatives of neuro-mesodermal progenitors via BMP-dependent Osr1 function. Dev Biol. 2021 Oct;478:155-162
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7286068
Gene symbol:	Wt1
Gene name:	WT1 transcription factor

Antibody:	anti-WT1 (C-19; sc-192)
Detection system:	Secondary antibody

Results

2K ©

Specimen 2K: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: metanephric mesenchyme	Present		2K

2L ©

Specimen 2L: embryonic day 10.5; Tbx6^tm1Pa/Tbx6^tm1Pa (more

)

Structure	Level	Pattern	Image	Note
TS17: metanephric mesenchyme	Absent		2L

3A ©

Specimen 3A: embryonic day 9.5 (more

)
Note: Forelimb level. Triple labeled: red - Wt1; white - Cdh1 (assay MGI:7286081); green - Sox2 (assay MGI:7286078).

Structure	Level	Pattern	Image	Note
TS15: mesonephros mesenchyme	Present		3A

3B ©

Specimen 3B: embryonic day 9.5; Tbx6^tm1Pa/Tbx6^tm1Pa (more

)
Note: Forelimb level. Triple labeled: red - Wt1; white - Cdh1 (assay MGI:7286081); green - Sox2 (assay MGI:7286078).

Structure	Level	Pattern	Image	Note
TS15: mesonephros mesenchyme	Present		3B	Wt1-expressing cells and ectopic Sox2-expressing cells were intermingled.

3E ©

Specimen 3E: embryonic day 9.5 (more

)
Note: Hindlimb level. Triple labeled: red - Wt1; white - Cdh1 (assay MGI:7286081); green - Sox2 (assay MGI:7286078).

Structure	Level	Pattern	Image	Note
TS15: mesonephros mesenchyme	Present		3E

3F ©

Specimen 3F: embryonic day 9.5; Tbx6^tm1Pa/Tbx6^tm1Pa (more

)
Note: Hindlimb level. Triple labeled: red - Wt1; white - Cdh1 (assay MGI:7286081); green - Sox2 (assay MGI:7286078).

Structure	Level	Pattern	Image	Note
TS15: mesonephros mesenchyme	Present	Regionally restricted	3F	At the hindlimb level, Wt1 was not expressed, suggesting a defect in the posterior extension. Ectopic Sox2 was expressed in an open tube-like structure.

4A ©

Specimen 4A: embryonic day 9.5 (more

)
Note: Double labeled: red - Wt1; green - Cdh1 (assay MGI:7286082).

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present		4A
TS15: mesonephros mesenchyme	Present		4A

4B ©

Specimen 4B: embryonic day 9.5; Osr1^em1Tkto/Osr1^em1Tkto (more

)
Note: Double labeled: red - Wt1; green - Cdh1 (assay MGI:7286082).

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from splanchnopleure	Present		4B
TS15: mesonephros mesenchyme	Absent		4B

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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