Gbx2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:312419 Haddad-Tovolli R, et al., Mouse thalamic differentiation: gli-dependent pattern and gli-independent prepattern. Front Neurosci. 2012;6:27
Assay type:	RNA in situ
MGI Accession ID:	MGI:7284816
Gene symbol:	Gbx2
Gene name:	gastrulation brain homeobox 2

Probe:	Gbx2 probe24
Probe preparation:	Antisense RNA

Results

1L ©

Specimen 1L: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present		1L

1M ©

Specimen 1M: embryonic day 12.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS20: thalamus	Weak		1M	Expression was very reduced in extension and intensity.

1N ©

Specimen 1N: embryonic day 12.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present		1N

2M ©

Specimen 2M: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: midline thalamic group	Present	Regionally restricted	2M	Expressed in the mediodorsal, medioventral, centrolateral and centromedial nuclei.

2N ©

Specimen 2N: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: geniculate thalamic group	Present	Regionally restricted	2N	Expressed in the medial geniculate nucleus.

2O ©

Specimen 2O: embryonic day 18.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS26: midline thalamic group	Present		2O	Severely reduced expression, but can still be detected in a midline domain.

2P ©

Specimen 2P: embryonic day 18.5; Gli3^Xt/Gli3^Xt (more

)

Structure	Level	Pattern	Image	Note
TS26: geniculate thalamic group	Absent		2P

2Q ©

Specimen 2Q: embryonic day 18.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS26: midline thalamic group	Present		2Q	Similar as in the wild-type (summarized in Table1).

2R ©

Specimen 2R: embryonic day 18.5; Gli2^tm1Alj/Gli2^tm1Alj (more

)

Structure	Level	Pattern	Image	Note
TS26: geniculate thalamic group	Present	Regionally restricted	2R	Very reduced expression in the medial geniculate nucleus (summarized in Table1).

5M ©

Specimen 5M: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: midline thalamic group	Present	Regionally restricted	5M	Expressed in the mediodorsal, medioventral, centrolateral and centromedial nuclei.

5N ©

Specimen 5N: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: geniculate thalamic group	Present	Regionally restricted	5N	Expressed in the medial geniculate nucleus.

5O ©

Specimen 5O: embryonic day 18.5; Foxb1^tm1(cre)Gabo/Foxb1⁺, Shh^tm2Amc/Shh^tm2Amc, Gli3^Xt/Gli3^Xt (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: midline thalamic group	Present	Regionally restricted	5O	Expressed in a wide ventral band.

5P ©

Specimen 5P: embryonic day 18.5; Foxb1^tm1(cre)Gabo/Foxb1⁺, Shh^tm2Amc/Shh^tm2Amc, Gli3^Xt/Gli3^Xt (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: thalamic nucleus	Weak	Regionally restricted	5P	Expressed as two dorsal stripes.

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