Gene Expression Data
Assay Details
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Assay
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Reference:
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J:300754
Shan Y, et al., Drebrin regulates cytoskeleton dynamics in migrating neurons through interaction with CXCR4. Proc Natl Acad Sci U S A. 2021 Jan 19;118(3):e2009493118
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7281736
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Antibody:
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anti-GnRH
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixative was 4% formaldehyde.
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Results
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Specimen
1C:
embryonic day 12.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Present |
Regionally restricted |
1C
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Expression is in migrating neurons.
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Specimen
not shown E12.5 WT:
embryonic day 12.5
(more )
Specimen
not shown E12.5 WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Specimen
not shown PN4 WT:
postnatal day 4
(more )
Specimen
not shown PN4 WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Specimen
not shown Adult WT:
postnatal adult
(more )
Specimen
not shown Adult WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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Specimen
not shown E12.5 KO:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Dbn1Gt(OST7352)Lex/Dbn1Gt(OST7352)Lex
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forebrain |
Weak |
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Significantly fewer positive cells (34%) were observed in this mutant in the nasal forebrain junction compared to wild-type.
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Specimen
not shown PN4 KO:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Dbn1Gt(OST7352)Lex/Dbn1Gt(OST7352)Lex
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: forebrain |
Present |
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In this mutant the total positive cell numberis are similar to wild-type but the mutant had more positive neurons rostral to the organulum vasculosum lamina terminalis.
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Specimen
not shown Adult KO:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Dbn1Gt(OST7352)Lex/Dbn1Gt(OST7352)Lex
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: forebrain |
Present |
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In this mutant the total positive cell numberis are similar to wild-type but the mutant had more positive neurons rostral to the organulum vasculosum lamina terminalis.
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Analysis Tools