Gene Expression Data
Assay Details
Assay
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Reference:
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J:227060
Fujikawa K, et al., An in situ hybridization study of perlecan, DMP1, and MEPE in developing condylar cartilage of the fetal mouse mandible and limb bud cartilage. Eur J Histochem. 2015;59(3):2553
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7280937
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Gene symbol:
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Hspg2
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Gene name:
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perlecan (heparan sulfate proteoglycan 2)
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Probe:
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Perlecan probe3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2b:
embryonic day 18.5
(more )
Specimen
2b:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: mandible |
osteocyte |
Present |
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2b
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Expression in many osteocytes.
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Specimen
3b:
embryonic day 14.5
(more )
Specimen
3b:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3f:
embryonic day 15.5
(more )
Specimen
3f:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3j:
embryonic day 16.5
(more )
Specimen
3j:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: mandible condylar process |
chondrocyte |
Present |
Regionally restricted |
3j
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Expression in chondrocytes from flattened cell zone to upper hypertrophic cell zone, reduced expression in lower hypertrophic cell zone.
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Specimen
4b:
embryonic day 12.5
(more )
Specimen
4b:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: lower leg mesenchyme |
Absent |
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4b
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No expression in mesenchymal cell condensation of anlage of future tibia.
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Specimen
4f:
embryonic day 13.5
(more )
Specimen
4f:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4j:
embryonic day 14.5
(more )
Specimen
4j:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: tibia cartilage condensation |
chondrocyte |
Present |
Regionally restricted |
4j
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Expression in chondrocytes throughout entire proliferative and maturation cells zones; weaker expression in hypertrophic cell zone.
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