Atoh1 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:313342 Nichols DH, et al., Interaction with ectopic cochlear crista sensory epithelium disrupts basal cochlear sensory epithelium development in Lmx1a mutant mice. Cell Tissue Res. 2020 Jun;380(3):435-448
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7279130
Gene symbol:	Atoh1
Gene name:	atonal bHLH transcription factor 1

Reporter:	lacZ
	Direct Detection

Results

Specimen 2a: embryonic day 17.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)

Structure	Level	Pattern	Image	Note
TS25: semicircular canal epithelium	Present	Regionally restricted	2a	Expressed in sensory epithelium at the site occupied by the Sox2-expressing cochlear crista sensory epithelium the previous day. Expressed in larger patches proximally and progressively smaller patches distally.

Specimen 2a': embryonic day 17.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS25: semicircular canal epithelium		Present	Regionally restricted	2a'	Expressed in sensory epithelium at the site occupied by the Sox2-expressing cochlear crista sensory epithelium the previous day. The cochlear crista ended in scattered cells and the previously associated basal cochlear sensory epithelium retained patches of outer hair cells.
TS25: cochlear duct epithelium	cochlear outer hair cell	Present		2a'

Specimen 2b: embryonic day 18.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)
Note: Embedded in epoxy resin.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	Present		2b
TS26: crista ampullaris	Present	Regionally restricted	2b	Expressed in cochlear crista.

Specimen 4a: postnatal day 1.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)

Structure	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	Present		4a
TS27: crista ampullaris	Present	Regionally restricted	4a	Expressed in cochlear crista parted from the basal cochlear sensory epithelium.

Specimen 4b: postnatal day 1.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)
Note: Embedded in epoxy resin.

Structure	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	Present	Regionally restricted	4b	Expressed in the basal cochlear sensory epithelium.
TS27: macula of saccule	Present		4b

Specimen 4h: embryonic day 17.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)

Structure	Level	Pattern	Image	Note
TS25: macula of saccule	Present		4h
TS25: utricle	Present		4h
TS25: semicircular canal epithelium	Present	Regionally restricted	4h	Expressed in anterior, horizontal, and cochlear cristae. The cochlear crista and medial arm of the posterior crista still parallel the basal cochlear sensory epithelium they previously contacted.
TS25: cochlear duct epithelium	Present		4h	The basal region of the cochlear sensory epithelium was not in the cochlear duct, but on the medial wall of the common vestibular cavity.

Specimen 5c: embryonic day 17.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)

Structure	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	Present	Regionally restricted	5c	Expressed in the basal cochlear sensory epithelium.
TS25: semicircular canal epithelium	Present	Regionally restricted	5c	Expressed in cochlear crista.
TS25: cochlear duct	Present	Scattered	5c	Scattered positive hair cells were occasionally seen.

Specimen 6a: embryonic day 17.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	cochlear inner hair cell	Present		6a	The basal cochlear sensory epithelium of the mutant completely lacked an outer hair cell band, and the inner band was variably expanded to several hair cells wide.
TS25: cochlear duct epithelium	cochlear outer hair cell	Present		6a	The basal cochlear sensory epithelium of the mutant completely lacked an outer hair cell band, and the inner band was variably expanded to several hair cells wide.

Specimen 6b: embryonic day 18.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	cochlear inner hair cell	Present		6b	The basal cochlear sensory epithelium of the mutant completely lacked an outer hair cell band, and the inner band was variably expanded to several hair cells wide.
TS26: cochlear duct epithelium	cochlear outer hair cell	Present		6b	The basal cochlear sensory epithelium of the mutant completely lacked an outer hair cell band, and the inner band was variably expanded to several hair cells wide.

Specimen 6d: embryonic day 18.5; Atoh1^tm2Hzo/Atoh1⁺, Lmx1a^dr-6J/Lmx1a^dr-6J (more

)
Note: Embedded in epoxy resin.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct epithelium	Present		6d	The basal cochlear sensory epithelium of the mutant completely lacked an outer hair cell band, and the inner band was variably expanded to several hair cells wide.

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