Probe:	47A1
Probe preparation:	Antisense labelled with P33
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: decidua	Very strong		1B	Extrememly high levels of expression in maternally derived decidual tissues surrounding embryo.

Structure	Level	Pattern	Image	Note
TS11: primitive endoderm	Present		1B	Lesser expression only in visceral endoderm.
TS11: embryo	Absent		1B
TS11: extraembryonic mesoderm	Absent		1B
TS11: parietal endoderm	Absent		1B
TS11: trophectoderm	Absent		1B

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		2B
TS15: bulbus cordis	Present	Diffuse	2B
TS15: heart atrium	Present	Diffuse	2B
TS15: primitive ventricle	Present	Diffuse	2B
TS15: branchial arch	Present	Regionally restricted	2B	Expression at surface of branchial arch.

Structure	Level	Pattern	Image	Note
TS11: yolk sac endoderm	Present		3B	Expression in visceral endoderm.
TS11: yolk sac mesoderm	Absent		3B

Structure	Level	Pattern	Image	Note
TS15: yolk sac endoderm	Present		3D	Expression in visceral endoderm.
TS15: yolk sac mesenchyme	Absent		3D

Structure	Level	Pattern	Image	Note
TS22: yolk sac endoderm	Present		3F	Expression in visceral endoderm.
TS22: yolk sac mesenchyme	Absent		3F

Structure	Level	Pattern	Image	Note
TS24: heart	Strong		4B	Highest levels of expression in yolk sac, epidermis, heart and intestine.
TS24: intestine	Strong		4B	Highest levels of expression in yolk sac, epidermis, heart and intestine.
TS24: yolk sac endoderm	Strong		4B	Highest levels of expression in yolk sac, epidermis, heart and intestine.
TS24: epidermis	Strong	Regionally restricted	4B	Highest level of expression in embryo proper within ventral epidermis.
TS24: trunk skin	Present	Graded	4B	Epidermis expression in torso occurs in a ventral to dorsal gradient.
TS24: limb epidermis	Present	Graded	4B	Epidermis expression in developing paw occurs in a dorsal to ventral gradient
TS24: heart atrium	Strong		4B	Expression greater in atrium than in ventricle.
TS24: heart ventricle	Present		4B	Expression greater in atrium than in ventricle.
TS24: basioccipital bone	Weak	Regionally restricted	4B	Low expression in ossification centers in jaw, head and spine.
TS24: basisphenoid bone	Weak	Regionally restricted	4B	Low expression in ossification centers in jaw, head and spine.
TS24: clavicle	Weak	Regionally restricted	4B	Low expression in ossification centers in jaw, head and spine.
TS24: mandible	Weak	Regionally restricted	4B	Low expression in ossification centers in jaw, head and spine.
TS24: rib	Weak	Regionally restricted	4B	Low expression in ossification centers in jaw, head and spine.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Absent		4C	No expression in epidermis of snout region.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Strong	Regionally restricted	4E	Marked expression in epidermis of snout region.

Structure	Level	Pattern	Image	Note
TS24: lung vascular element	Present	Regionally restricted	5A	Expression in cells surrounding some blood vessels but not others. Expression most evident lining larger blood vessels.

Structure	Level	Pattern	Image	Note
TS24: pulmonary vein	Strong	Regionally restricted	5C	Highest expression in musculature surrounding pulmonary veins.

Structure	Level	Pattern	Image	Note
TS24: nephrogenic interstitium	Weak	Regionally restricted	5E	Low level of expression in interstitial cells.
TS24: cortical early proximal tubule	Present		5E	Expression in presumptive proximal tubule cells.

Structure	Level	Pattern	Image	Note
TS24: intestinal villus	Present	Graded	5G	Significant expression in intestinal villus with gradient of increasing exprssion between crypt and villus tuft.

Structure	Level	Pattern	Image	Note
TS28: kidney outer cortex	Strong		8B
TS28: renal cortex	Present	Regionally restricted	8B	Lower levels of expression in inner renal cortex.
TS28: renal medulla	Absent		8B

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Absent		8E

Structure	Level	Pattern	Image	Note
TS28: adipose tissue	Present		9B
TS28: heart atrium	Present		9B
TS28: heart ventricle	Absent		9B

Structure	Level	Pattern	Image	Note
TS28: atrium cardiac muscle	Present		9D
TS28: ventricle cardiac muscle	Absent		9D

Structure	Level	Pattern	Image	Note
TS28: brown fat	Present	Regionally restricted	9F	Significant expression in brown fat of mediastinum.