Pou5f1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:190555 Yoon Y, et al., Conditional Aurora A deficiency differentially affects early mouse embryo patterning. Dev Biol. 2012 Nov 1;371(1):77-85
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7264808
Gene symbol:	Pou5f1
Gene name:	POU domain, class 5, transcription factor 1

Antibody:	anti-Oct4 antibody (ab19857)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

1D Control ©

Specimen 1D Control: embryonic day 6.5; Aurka^tm1.1Tvd/Aurka⁺, Edil3^{Tg(Sox2-cre)1Amc}/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		1D Control	Expression in epiblast.

1D Epi-KO ©

Specimen 1D Epi-KO: embryonic day 6.5; Aurka^tm1.1Tvd/Aurka^tm1.2Tvd, Edil3^{Tg(Sox2-cre)1Amc}/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Regionally restricted	1D Epi-KO	Expression domain smaller than in control embryo.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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