Antibody:	anti-cleaved caspase-3 (Asp175) (5A1E; #9664)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS23: retina layer	Absent		5A	No apoptotic cells were observed in the neuroblast cell layer or the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS23: retina layer	Absent		5B	No apoptotic cells were observed in the neuroblast cell layer or the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS25: retina layer	Present	Regionally restricted	5C	Apoptotic cells were detected in the ganglion cell layer. No apoptotic cells were observed in the neuroblast cell layer.

Structure	Level	Pattern	Image	Note
TS25: retina layer	Present	Regionally restricted	5D	Apoptotic cells were detected in the ganglion cell layer. The number was signficantly increased in the mutant compared with wild type littermates. No apoptotic cells were observed in the neuroblast cell layer.

Structure	Level	Pattern	Image	Note
TS28: retina	Absent		5E	No apoptotic cells were observed in the retina.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		5F	Apoptotic cells were detected.
TS28: retina inner nuclear layer	Present		5F	Apoptotic cells were detected.
TS28: retina outer nuclear layer	Absent		5F	No apoptotic cells were observed.

Structure	Level	Pattern	Image	Note
TS28: retina	Absent		5G	No apoptotic cells were observed.

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present		5H	Apoptotic cells were detected.