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Gene Expression Data
Assay Details
Assay
Reference: J:312489 Tang W, et al., Hlf Expression Marks Early Emergence of Hematopoietic Stem Cell Precursors With Adult Repopulating Potential and Fate. Front Cell Dev Biol. 2021;9:728057
Assay type: Recombinase reporter
MGI Accession ID: MGI:7262871
Gene symbol: Hlf
Gene name: hepatic leukemia factor
Reporter: ZsGreen
Direct Detection
Assay notes: Single-dose of induction by tamoxifen injection was performed at E9.5 and dissociated cells were analyzed and sorted by FACS.
Results
Specimen Fig4E E15.5: embryonic day 15.5; Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/Gt(ROSA)26Sor+, Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS23: hematopoietic system hematopoietic precursor cell Present 4E cells in the immunophenotypic HSCs (Lin-,Sca-1+,Mac-1lo,CD201C+)
TS23: liver hematopoietic precursor cell Present 4E cells in the immunophenotypic HSCs (Lin-,Sca-1+,Mac-1lo,CD201C+)
Specimen Fig4D E11.5: embryonic day 11.5; Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/Gt(ROSA)26Sor+, Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS19: dorsal aorta hematopoietic precursor cell Present 4D
TS19: hematopoietic system hematopoietic precursor cell Present 4D
Specimen Fig4D E13.5: embryonic day 13.5; Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/Gt(ROSA)26Sor+, Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS21: hematopoietic system hematopoietic precursor cell Present 4D
TS21: liver hematopoietic precursor cell Present 4D
Specimen Fig4D E15.5: embryonic day 15.5; Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/Gt(ROSA)26Sor+, Hlfem1(cre/ERT2)Smoc/Hlf+ (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS23: hematopoietic system hematopoietic precursor cell Present 4D
TS23: liver hematopoietic precursor cell Present 4D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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