H19 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:308869 Szabo PE, et al., The chicken beta-globin insulator element conveys chromatin boundary activity but not imprinting at the mouse Igf2/H19 domain. Development. 2002 Feb;129(4):897-904
Assay type:	RT-PCR
MGI Accession ID:	MGI:7262665
Gene symbol:	H19
Gene name:	H19, imprinted maternally expressed transcript

Probe:	H19-pI2, H19-pJ2
Assay notes:	This is a SNuPE assay to to determine the relative proportions of maternal and paternal allele-specific RNA in hybrid embryos.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
+/-(P)	E18.5	TS26: liver	Present (a)	Not Specified	involves: 129S1/Sv * CAST/Ei * FVB/NJ * Swiss	Rr27^tm1.1Mnn/Rr27⁺	Not Specified	Paternally inherited mutant allele.
+/-(P)	E18.5	TS26: liver	Present (b)	Not Specified	involves: 129S1/Sv * CAST/Ei * FVB/NJ * Swiss	Rr27^tm1.1Mnn/Rr27⁺	Not Specified	Paternally inherited mutant allele.
+/+	E18.5	TS26: liver	Present	Not Specified	Not Specified		Not Specified
+/+	E18.5	TS26: liver	Present	Not Specified	Not Specified		Not Specified
-(M)/+	E18.5	TS26: liver	Present (c)	Not Specified	involves: 129S1/Sv * CAST/Ei * FVB/NJ * Swiss	Rr27^tm1.1Mnn/Rr27⁺	Not Specified	Maternally inherited mutant allele.
-(M)/+	E18.5	TS26: liver	Present (c)	Not Specified	involves: 129S1/Sv * CAST/Ei * FVB/NJ * Swiss	Rr27^tm1.1Mnn/Rr27⁺	Not Specified	Maternally inherited mutant allele.
+/-(P)	E18.5	TS26: metanephros	Present (d)	Not Specified	involves: 129S1/Sv * CAST/Ei * FVB/NJ * Swiss	Rr27^tm1.1Mnn/Rr27⁺	Not Specified	Paternally inherited mutant allele.
+/-(P)	E18.5	TS26: metanephros	Present (e)	Not Specified	involves: 129S1/Sv * CAST/Ei * FVB/NJ * Swiss	Rr27^tm1.1Mnn/Rr27⁺	Not Specified	Paternally inherited mutant allele.
+/+	E18.5	TS26: metanephros	Present	Not Specified	Not Specified		Not Specified
+/+	E18.5	TS26: metanephros	Present	Not Specified	Not Specified		Not Specified
-(M)/+	E18.5	TS26: metanephros	Present (c)	Not Specified	involves: 129S1/Sv * CAST/Ei * FVB/NJ * Swiss	Rr27^tm1.1Mnn/Rr27⁺	Not Specified	Maternally inherited mutant allele.
-(M)/+	E18.5	TS26: metanephros	Present (c)	Not Specified	involves: 129S1/Sv * CAST/Ei * FVB/NJ * Swiss	Rr27^tm1.1Mnn/Rr27⁺	Not Specified	Maternally inherited mutant allele.

Notes:
(a) There is biallelic expression. The paternal allele was activated to a level greater than that of the maternal allele, accounting for 76% of the total RNA.
(b) There is biallelic expression. The paternal allele was activated to a level greater than that of the maternal allele, accounting for 78% of the total RNA
(c) The maternal mutant allele was hypomethylated.
(d) There is biallelic expression. The paternal allele was activated to a level greater than that of the maternal allele, accounting for 74% of the total RNA
(e) There is biallelic expression. The paternal allele was activated to a level greater than that of the maternal allele, accounting for 74% of the total RNA

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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