Atp2a1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:309086 Jin S, et al., Ebf factors and MyoD cooperate to regulate muscle relaxation via Atp2a1. Nat Commun. 2014 May 2;5:3793
Assay type:	Western blot
MGI Accession ID:	MGI:7261207
Gene symbol:	Atp2a1
Gene name:	ATPase, Ca++ transporting, cardiac muscle, fast twitch 1

Antibody:	anti-Serca1
Detection system:	Secondary antibody coupled to alkaline phosphatase

Results

Image: 3H

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	110 kDa	Genetic Background	Mutant Allele(s)	Sex
Ebf3+/+ SM	E18.5	TS26: skeletal muscle	Present	Not Specified		Not Specified
Ebf3+/+ D	E18.5	TS26: diaphragm	Present	Not Specified		Not Specified
Ebf3+/- SM	E18.5	TS26: skeletal muscle	Present	B6.Cg-Ebf3^tm1.1Uhg	Ebf3^tm1.1Uhg/Ebf3⁺	Not Specified
Ebf3+/- D	E18.5	TS26: diaphragm	Present	B6.Cg-Ebf3^tm1.1Uhg	Ebf3^tm1.1Uhg/Ebf3⁺	Not Specified
Ebf3-/- SM	E18.5	TS26: skeletal muscle	Present (a)	B6.Cg-Ebf3^tm1.1Uhg	Ebf3^tm1.1Uhg/Ebf3^tm1.1Uhg	Not Specified
Ebf3-/- D	E18.5	TS26: diaphragm	Trace (b)	B6.Cg-Ebf3^tm1.1Uhg	Ebf3^tm1.1Uhg/Ebf3^tm1.1Uhg	Not Specified

Notes:
(a) There was no significant difference in the amount of protein in this tissue between mutant and wild-type.
(b) Quantification of the protein levels indicated a reduction by a factor of 3.2 compared to wild-type in this tisue.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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