Gene Expression Data
Assay Details
Assay
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Reference:
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J:308673
Company V, et al., Netrin 1-Mediated Role of the Substantia Nigra Pars Compacta and Ventral Tegmental Area in the Guidance of the Medial Habenular Axons. Front Cell Dev Biol. 2021;9:682067
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7259913
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Gene symbol:
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Robo3
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Gene name:
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roundabout guidance receptor 3
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Results
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Specimen
2K:
embryonic day 16.5
(more )
Note: Double labeled: purple - Robo3; green/white - Th (assay MGI:7259909).
Specimen
2K:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Robo3; green/white - Th (assay MGI:7259909).
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Structure |
Level |
Pattern |
Image |
Note |
TS24: fasciculus retroflexus |
Present |
Regionally restricted |
2K
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Expressed in the medial habenular axons of the fasciculus retroflexus.
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Specimen
2L:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Robo3; green/white - Th (assay MGI:7259909).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: fasciculus retroflexus |
Present |
Regionally restricted |
2L
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The medial habenular axons of the fasciculus retroflexus striking misdirection toward rostral domains were seen; no single axon was detected toward their caudal target. The misrouted tract seemed related to an enlarged A13 dopaminergic population located in the prethalamic territory.
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Specimen
2M:
embryonic day 16.5
(more )
Note: Double labeled: purple - Robo3; green/white - Th (assay MGI:7259909).
Specimen
2M:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Robo3; green/white - Th (assay MGI:7259909).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: fasciculus retroflexus |
Present |
Regionally restricted |
2M
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Expressed in the medial habenular axons of the fasciculus retroflexus in the pial surface.
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Specimen
2N:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Robo3; green/white - Th (assay MGI:7259909).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: fasciculus retroflexus |
Present |
Regionally restricted |
2N
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The medial habenular axons of the fasciculus retroflexus was misoriented and crosses the midline in an abnormal position, far away from the superficial side of the midbrain.
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