Gene Expression Data
Assay Details
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Assay
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Reference:
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J:168038
Bimonte S, et al., Ofd1 is required in limb bud patterning and endochondral bone development. Dev Biol. 2011 Jan 15;349(2):179-91
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7258470
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Gene symbol:
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Gli1
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Gene name:
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GLI-Kruppel family member GLI1
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Probe:
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Gli1 probe50
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
7G:
embryonic day 16.5
(more  )
Specimen
7G:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J * SJL/J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
columnar chondrocyte |
Present |
Regionally restricted |
7G
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Expression in columnar and prehypertrophic chondrocytes.
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| TS24: humerus |
growth plate cartilage chondrocyte |
Present |
Regionally restricted |
7G
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Expression in columnar and prehypertrophic chondrocytes.
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| TS24: perichondrium |
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Present |
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7G
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Specimen
7H:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J * SJL/J
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Mutant Allele(s):
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Ofd1tm2.1Bfra/Ofd1+
Tg(Prrx1-cre)1Cjt/0
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Age:
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embryonic day 16.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
columnar chondrocyte |
Present |
Regionally restricted |
7H
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Expression in columnar and prehypertrophic chondrocytes.
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| TS24: perichondrium |
growth plate cartilage chondrocyte |
Weak |
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7H
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Expression in perichondrium. significantly reduced compared with wildtype.
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Specimen
7I:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J * SJL/J
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Mutant Allele(s):
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Ofd1tm2.1Bfra/Y
Tg(Prrx1-cre)1Cjt/0
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
columnar chondrocyte |
Present |
Regionally restricted |
7I
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Expression in columnar and prehypertrophic chondrocytes.
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| TS24: humerus |
growth plate cartilage chondrocyte |
Present |
Regionally restricted |
7I
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Expression in columnar and prehypertrophic chondrocytes.
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| TS24: perichondrium |
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Trace |
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7I
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Expression almost absent in perichondrium of male mutants.
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Analysis Tools