Probe:	Calm2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	1D	Expressed in the posterior extramural stream, which crossed the midline and reached the contralateral prospective lateral reticular nucleus region. Expression was relatively low compared with its surroundings.
TS22: hindbrain	Present		1D	Expressed in most hindbrain areas, with relatively strong expression in the brainstem motor nuclei.
TS22: brainstem nucleus	Present		1D
TS22: hindbrain floor plate	Absent		1D

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	1I	Expressed in the anterior extramural stream, which just reached the ipsilateral prospective pontine nucleus region.
TS22: brainstem nucleus	Present		1I
TS22: hindbrain	Present		1I	Expressed in most hindbrain areas, with relatively strong expression in the brainstem motor nuclei.
TS22: hindbrain floor plate	Absent		1I

Structure	Level	Pattern	Image	Note
TS25: medulla oblongata	Present	Regionally restricted	1N	Expressed in the posterior extramural stream, which gives rise to the external cuneate nucleus.

Structure	Level	Pattern	Image	Note
TS25: medulla oblongata	Present	Regionally restricted	1R	Expressed in the posterior extramural stream, which gives rise to the lateral reticular nucleus.

Structure	Level	Pattern	Image	Note
TS25: pons	Present	Regionally restricted	1V	Expressed in the anterior extramural stream, which gives rise to the pontine nucleus.