About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:182768 Wong YL, et al., Smad1/Smad5 signaling in limb ectoderm functions redundantly and is required for interdigital programmed cell death. Dev Biol. 2012 Mar 1;363(1):247-57
Assay type: RNA in situ
MGI Accession ID: MGI:7257795
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Results
Specimen 4A: embryonic day 10.5; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1+, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 4A
Specimen 4B: embryonic day 10.5; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1tm1Abr, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 4B Similar pattern of expression as in controls. No expansion of expression along dorsal-ventral or anterior-posterior axes.
Specimen 4C: embryonic day 11.5; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1+, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 4C
Specimen 4D: embryonic day 11.5; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1tm1Abr, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 4D Expression along dorsal-ventral axis comparable with control.
Specimen 4G: embryonic day 13.0; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1+, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb digit epithelium Present Regionally restricted 4G Expression in AER overlying developing digits.
TS21: forelimb interdigital region epithelium Absent 4G Expression ceased over interdigital region
Specimen 4H: embryonic day 13.0; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1tm1Abr, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb interdigital region epithelium Present Regionally restricted 4H Expression maintained in AER over interdigital region.
TS21: hand epithelium Present Punctate 4H Punctate expression throughout distal AER.
Specimen 4I: embryonic day 13.0; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1+, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb digit epithelium Present Regionally restricted 4I Expression in AER overlying developing digits.
TS21: forelimb interdigital region epithelium Absent 4I Expression ceased over interdigital region.
Specimen 4J: embryonic day 13.0; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1tm1Abr, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb interdigital region epithelium Present Regionally restricted 4J Expression maintained in AER over interdigital region.
TS21: hand epithelium Present Punctate 4J Punctate expression throughout distal AER.
Specimen 4K: embryonic day 13.5; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1+, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb interdigital region epithelium Ambiguous 4K
Specimen 4L: embryonic day 13.5; En1tm2(cre)Wrst/En1+, Smad1tm1Abr/Smad1tm1Abr, Smad5tm1Huy/Smad5tm1Huy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: forelimb interdigital region epithelium Absent 4L Expression faded/ceased in AER over interdigital region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
01/06/2026
MGI 6.24 		The Jackson Laboratory