Mki67 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:180785 Loebel DA, et al., Regionalized Twist1 activity in the forelimb bud drives the morphogenesis of the proximal and preaxial skeleton. Dev Biol. 2012 Feb 15;362(2):132-40
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7256598
Gene symbol:	Mki67
Gene name:	antigen identified by monoclonal antibody Ki 67

Antibody:	anti-Ki67
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

2O Ki67 ©

2O merge ©

Specimen 2O: embryonic day 9.5; Twist1^tm2Bhr/Twist1⁺ (more

)
Note: Triple labeled: red- Mki67; green - Twist1 (assay MGI:7256597); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		2O merge , 2O Ki67

2P Ki67 ©

2P merge ©

Specimen 2P: embryonic day 9.5; Mesp1^tm2(cre)Ysa/Mesp1⁺, Twist1^tm2Bhr/Twist1^tm2.1Bhr (more

)
Note: Conditional mutant. Triple labeled: red- Mki67; green - Twist1 (assay MGI:7256597); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Weak		2P merge , 2P Ki67	Lower frequency of expressing cells than in wild type limb bud.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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