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Gene Expression Data
Assay Details
Assay
Reference: J:180785 Loebel DA, et al., Regionalized Twist1 activity in the forelimb bud drives the morphogenesis of the proximal and preaxial skeleton. Dev Biol. 2012 Feb 15;362(2):132-40
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7256597
Gene symbol: Twist1
Gene name: twist basic helix-loop-helix transcription factor 1
Antibody: anti-Twist1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2O: embryonic day 9.5; Twist1tm2Bhr/Twist1+ (more )
Note: Triple labeled: green - Twist1; red - Mki67 (assay MGI:7256598); blue - DAPI.
Structure Level Pattern Image Note
TS15: forelimb bud Present Regionally restricted 2O Twist1 , 2O merge Region of non-expressing cells in proximal mesenchyme iwth a greater concentration of these cells towards anterior of limb bud.
Specimen 2P: embryonic day 9.5; Mesp1tm2(cre)Ysa/Mesp1+, Twist1tm2Bhr/Twist1tm2.1Bhr (more )
Note: Conditional mutant. Triple labeled: green - Twist1; red - Mki67 (assay MGI:7256598); blue - DAPI.
Structure Level Pattern Image Note
TS15: forelimb bud Present Regionally restricted 2P merge , 2P Twist1 Region of non-expressing cells in proximal mesenchyme iwth a greater concentration of these cells towards anterior of limb bud.
Specimen 2Q: embryonic day 10.5; Twist1tm2Bhr/Twist1+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2Q Twist1 , 2Q Twist DAPI Non-expressing cells mostly localized to proximal half of forelimb bud, mostly in anterior sector.
Specimen 2R: embryonic day 10.5; Mesp1tm2(cre)Ysa/Mesp1+, Twist1tm2Bhr/Twist1tm2.1Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted 2R Twist DAPI , 2R Twist1 Non-expressing cells mostly localized to proximal half of forelimb bud, mostly in anterior sector.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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