Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:199480 Cao T, et al., Mouse limbs expressing only the Gli3 repressor resemble those of Sonic hedgehog mutants. Dev Biol. 2013 Jul 15;379(2):221-8
Assay type:	RNA in situ
MGI Accession ID:	MGI:7256304
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe206
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

4H1 ©

Specimen 4H1: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		4H1

4H2 ©

Specimen 4H2: embryonic day 10.5; Gli3^tm3.1Blnw/Gli3⁺, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		4H2	Expression unaffected compared with wild type.

4H3 ©

Specimen 4H3: embryonic day 10.5; Gli3^tm3.1Blnw/Gli3^tm3.1Blnw, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		4H3	Expression unaffected compared with wild type.

4H4 ©

Specimen 4H4: embryonic day 10.5; Shh^tm1Chg/Shh^tm1Chg (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Absent		4H4

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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