Gene Expression Data
Assay Details
Assay
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Reference:
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J:320343
Randilini A, et al., An in situ hybridization study of decorin and biglycan mRNA in mouse osteoblasts in vivo. Anat Sci Int. 2021 Mar;96(2):265-272
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7254845
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Gene symbol:
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Dcn
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Gene name:
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decorin
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Probe:
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decorin probe2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1d:
embryonic day 13.5
(more )
Specimen
1d:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: mandible |
Present |
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1d
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Authors report no characteristic expression.
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Specimen
1i:
embryonic day 14.5
(more )
Specimen
1i:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: mandible |
osteoblast |
Present |
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1i
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Expression started in osteoblasts but differential expression hardly recognized.
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Specimen
2e:
embryonic day 15.5
(more )
Specimen
2e:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: maxilla |
osteoblast |
Present |
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2e
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Expression in osteoblasts around maxillary bone. Authors report differential expression pattern was hardly recognized.
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Specimen
3d:
embryonic day 14.5
(more )
Specimen
3d:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3i:
embryonic day 15.5
(more )
Specimen
3i:
(close )
Genetic Background:
|
ICR
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Age:
|
embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: tympanic ring |
osteoblast |
Present |
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3i
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Expression in osteoblasts around tympanic ring.
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Specimen
3n:
embryonic day 16.5
(more )
Specimen
3n:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: tympanic ring |
osteoblast |
Present |
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3n
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Expression on osteoblasts around tympanic ring.
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Specimen
4d:
embryonic day 14.5
(more )
Specimen
4d:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: mandible ramus |
osteoblast |
Present |
Regionally restricted |
4d
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Expression in osteoblasts around extending bone trabecula to the posterior end.
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Specimen
4i:
embryonic day 15.5
(more )
Specimen
4i:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: mandible ramus |
osteoblast |
Present |
Regionally restricted |
4i
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Mainly expressed in osteoblasts in perichondrium of mandibular ramus. Expression around around bone collar. Weak expression in endosteum (inner region) of mandibular ramus.
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