Gene Expression Data
Assay Details
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Assay
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Reference:
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J:301614
Town L, et al., Tmem26 is dynamically expressed during palate and limb development but is not required for embryonic survival. PLoS One. 2011;6(9):e25228
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:6890355
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Gene symbol:
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Tmem26
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Gene name:
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transmembrane protein 26
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Probe:
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Tmem26-pE, Tmem26-pF
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Assay notes:
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This assay is a summary of data not shown from the publication. The following statement appears in the text: To examine the transcriptional products of the Tmem26<Ex2-> allele, we used RT-PCR to amplify across the introduced mutation from mRNA derived from adult liver and brain. Two forward primers were designed within exon 1 and reverse primers were designed within exon 3, 4 and 5, thus allowing 6 separate PCR reactions that amplify regions that include exon 2. All PCR products were band purified and sequenced. Two Tmem26 transcripts were amplified specifically from Tmem26<Ex2-/Ex2-> tissues. Sequencing revealed that the larger of these bands represented the expected mutant transcript lacking exon 2. The smaller band lacked both exons 2 and 3, indicating that deletion of exon 2 induced a splice variant that contains exons 1, 4, 5 and 6. Fig. 5B shows a diagram of the observed transcripts in the mutant.
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Results
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Notes:
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Analysis Tools
