Probe:	IMAGE clone 3168716
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Three probes were used for whole mount in situ hybridization with similar results. MGI:1064176 was used for all of the results in this assay. The other probes were MGI:6185423 and MGI:6888159.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent

Structure	Level	Pattern	Image	Note
TS18: limb mesenchyme	Present	Regionally restricted	2A	Expression was detected in the mesenchyme of the anterior and posterior proximal limb bud, at the autopod/zeugopod boundary.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	2B	By E11.5, expression was increased in the domains from E11.0.
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	2B	By E11.5, expression was increased in the domains from E11.0.
TS19: trunk	Present	Regionally restricted	2B	Expression was detected in a broad domain across the surface of the flank.
TS19: head	Present		2B	Expression was detected in craniofacial structures.

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region mesenchyme	Present		2C	Expression was detected throughout much of the interdigital space mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	2D	By E13.5, expression was limited to the mesenchyme at the margins of the digits.
TS21: hand mesenchyme	Present	Regionally restricted	2D	By E13.5, expression was limited to the mesenchyme at the margins of the digits.

Structure	Level	Pattern	Image	Note
TS22: foot mesenchyme	Present	Regionally restricted	2E	Expression was limited to the mesenchyme at the margins of the digits.
TS22: hand mesenchyme	Present	Regionally restricted	2E	Expression was limited to the mesenchyme at the margins of the digits.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present	Regionally restricted	2F	Expression was detected in the mesenchyme of the anterior and posterior proximal limb bud, at the autopod/zeugopod boundary.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	2G	By E11.5, expression was increased in the domains from E11.0.

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region mesenchyme	Present		2H	Expression was detected throughout much of the interdigital space mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	2I	By E13.5, expression was limited to the mesenchyme at the margins of the digits.

Structure	Level	Pattern	Image	Note
TS22: hand mesenchyme	Present	Regionally restricted	2J	Expression was limited to the mesenchyme at the margins of the digits.

Structure	Level	Pattern	Image	Note
TS18: latero-nasal process	Present	Diffuse	2K
TS18: medial-nasal process	Present	Diffuse	2K

Structure	Level	Pattern	Image	Note
TS19: latero-nasal process mesenchyme	Present	Regionally restricted	2L	Expression was detected in the mesenchyme adjacent to the region of fusion to form the upper lip and primary palate.
TS19: maxillary process mesenchyme	Present	Regionally restricted	2L	Expression was detected in the mesenchyme adjacent to the region of fusion to form the upper lip and primary palate.
TS19: medial-nasal process mesenchyme	Present	Regionally restricted	2L	Expression was detected in the mesenchyme adjacent to the region of fusion to form the upper lip and primary palate.
TS19: maxillary process	Present	Regionally restricted	2L	Expression was detected along the posterior margin of the maxillary prominence.
TS19: medial-nasal process	Present	Regionally restricted	2L	Expression was detected along the medial surface of each medial nasal prominence near the region where they will ultimately merge at the midline.
TS19: mandibular process	Present	Regionally restricted	2L	Expression was detected along the oral edge of the mandibular prominence.

Structure	Level	Pattern	Image	Note
TS20: primary palate	Present		2M
TS20: mandibular process	Present	Regionally restricted	2M	Expression was detected along the oral edge of the mandibular prominence.

Structure	Level	Pattern	Image	Note
TS21: maxilla	Present	Regionally restricted	2N	Expression was restricted to the distal tips of the maxillae.
TS21: naris	Present	Regionally restricted	2N	Expression was detected in the maxillary margins of the nostrils.
TS21: mandible	Present	Regionally restricted	2N	Expression was detected along the oral edge of the mandibular prominence.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf	Present		2O	Expression was detected in the palatal shelves prior to fusion.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Weak	Regionally restricted	2P	Expression appeared to be downregulated in the neighboring mesenchyme as the regions of the palate began to fuse.

Structure	Level	Pattern	Image	Note
TS23: secondary palate	Trace		2Q

Structure	Level	Pattern	Image	Note
TS21: preputial swelling	Present		2R
TS21: genital tubercle	Present	Regionally restricted	2R	Expression was detected in the proximo-lateral glans of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS21: preputial swelling	Present		2S
TS21: genital tubercle	Present	Regionally restricted	2S	Expression was detected in the proximo-lateral glans of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Present	Regionally restricted		Expression was detected in the preputial swellings and proximo-lateral glans of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS23: glans of genital tubercle	Present
TS23: preputial swelling	Present