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Gene Expression Data
Assay Details
Assay
Reference: J:316590 Rybtsov S, et al., Tracing the origin of the HSC hierarchy reveals an SCF-dependent, IL-3-independent CD43(-) embryonic precursor. Stem Cell Reports. 2014 Sep 9;3(3):489-501
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6887670
Gene symbol: Runx1
Gene name: runt related transcription factor 1
Antibody: anti-RUNX1/AML1 + RUNX2 + RUNX3 (EPR3099; ab92336)
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3A: embryonic day 9.0 (more )
Note: Triple labeled: blue - Runx1; green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS14: dorsal aorta Present Regionally restricted 3A Merge , 3A Runx1 Expression in cells localized to ventral floor of dorsal aorta.
Specimen 3A': embryonic day 9.0 (more )
Note: Triple labeled: blue - Runx1; green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS14: dorsal aorta Present Regionally restricted 3A' Runx1 , 3A' Merge Expression in pro-hematopoietic stem cells (pro-HSCs) localized to ventral endothelium.
Specimen 3B: embryonic day 9.0 (more )
Note: Triple labeled: blue - Runx1; green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS14: vitelline artery Present Regionally restricted 3B Merge , 3B Runx1 Expression in strings of large clusters inside omphalomesenteric (vitelline) artery.
TS14: blood vessel Present Regionally restricted 3B Runx1 , 3B Merge Expression in small vessels connecting omphalomesenteric (vitelline) artery and dorsal aorta.
Specimen 3B': embryonic day 9.0 (more )
Note: Triple labeled: blue - Runx1; green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS14: vitelline artery Present Regionally restricted 3B' Merge , 3B' Runx1 Expression in large clusters inside omphalomesenteric artery.
Specimen 3E: embryonic day 10.0 (more )
Note: Triple labeled: blue - Runx1; green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS16: dorsal aorta Present Regionally restricted 3E Runx1 , 3E Merge Expression in intra-aortic clusters.
Specimen S3D: embryonic day 9.5 (more )
Note: Triple labeled: blue - Runx1; green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS15: hematopoietic system Present Regionally restricted S3D Merge , S3D Runx1 Expression in hematopoitic cells in circulation within dorsal aorta.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory