Gene Expression Data
Assay Details
Assay
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Reference:
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J:321365
Knoedler JR, et al., A functional cellular framework for sex and estrous cycle-dependent gene expression and behavior. Cell. 2022 Feb 17;185(4):654-671.e22
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6886052
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Gene symbol:
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Cckar
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Gene name:
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cholecystokinin A receptor
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Probe:
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Cckar probe1
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Probe preparation:
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Antisense labelled with Alexa Fluor
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Visualized with:
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Fluorescence
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Results
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Specimen
S1F:
postnatal adult
(more )
Note: coronal section
Specimen
S1F:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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coronal section
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: medial amygdaloid nucleus |
neuron |
Present |
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S1F Cckar
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Some expression is seen in the medial amygdala (MeA).
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Specimen
3C:
postnatal adult
(more )
Note: Double labeled, sexually receptive female: green - Cckar; red - Esr1 (assay MGI:6886064)
Specimen
3C:
(close )
Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
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Double labeled, sexually receptive female: green - Cckar; red - Esr1 (assay MGI:6886064)
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: medial amygdaloid nucleus |
neuron |
Present |
|
3C Cckar - DEG
,
3C Cckar - Merge
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Sparse expression is seen in the medial amygdala (MeA); 4.6% of Esr1 positive neurons cells express it.
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Specimen
7C:
postnatal adult
(more )
Note: Double labeled: red - Cckar; green - Esr1 (assay MGI:6886064)
Specimen
7C:
(close )
Genetic Background:
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C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
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Female
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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Double labeled: red - Cckar; green - Esr1 (assay MGI:6886064)
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: ventromedial hypothalamic nucleus |
neuron |
Present |
Regionally restricted |
7C Merge
,
7C Cckar
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Expression is restricted to the ventrolateral component of the Esr1 positive cells in the ventromedial hypothalamus ventrolateralis (VMHvl).
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Specimen
S7D:
postnatal adult; Cckarem1(cre)Shah/Cckar +
(more )
Note: Double labeled: green - Cckar RNA; red - Cckar reporter (assay MGI:6885930)
Specimen
S7D:
(close )
Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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Cckarem1(cre)Shah/Cckar+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Note:
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Double labeled: green - Cckar RNA; red - Cckar reporter (assay MGI:6885930)
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: ventromedial hypothalamic nucleus |
neuron |
Present |
Regionally restricted |
S7D Cckar
,
S7D Merge
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Expression is seen in the ventromedial hypothalamus ventrolateralis (VMHvl).
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