Gene Expression Data
Assay Details
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Assay
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Reference:
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J:137801
Nakayama A, et al., Expression of the basal cell markers of taste buds in the anterior tongue and soft palate of the mouse embryo. J Comp Neurol. 2008 Jul 10;509(2):211-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6885879
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe202
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 11.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tongue |
Present |
Diffuse |
1A
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Diffuse expression on dorsal surface of anterior tongue.
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Specimen
1B:
embryonic day 12.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: tongue papillary placode |
Present |
Regionally restricted |
1B
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Focused pattern of expression corresponding to distribution of fungiform papilla placodes.
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Specimen
4A:
embryonic day 13.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: soft palate |
Absent |
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4A
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No expression in prospective soft palatal region.
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| TS21: secondary palate |
Present |
Regionally restricted |
4A
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Broad band of expression in presumptive rugae in hard palatal region.
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Specimen
4B:
embryonic day 14.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: soft palate |
Present |
Punctate |
4B
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Expression in bands in prospective Geschmacksstreifen (GS); punctate pattern of expression posteriorly from prospective GS. Size of expressing spots varied.
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| TS22: secondary palate |
Present |
Regionally restricted |
4B
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Expression restricted to parasagittal region of palate.
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| TS22: palatal shelf epithelium |
Absent |
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4B
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Expression not observed in medial edge epithelium of palatal shelf.
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Specimen
E14.5 not shown:
embryonic day 14.5
(more )
Specimen
E14.5 not shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: secondary palate |
Present |
Regionally restricted |
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Bands of expression on presumptive rugae were increased to 6 or 7.
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Specimen
4C:
embryonic day 15.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: soft palate |
Strong |
Regionally restricted |
4C
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Increased intensity of expression and number of spots of expression. Spots not observed on fusion line in soft palatal region.
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Specimen
4D:
embryonic day 16.5
(more )
Specimen
4D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: soft palate |
Present |
Spotted |
4D
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Spots of expression observed in posterior midline of soft palate.
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Specimen
5A:
embryonic day 16.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: soft palate |
Present |
Spotted |
5A
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Expression in spots; no expression immediately surrounding spots of expression. Weak broad expression elsewhere.
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Analysis Tools