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Gene Expression Data
Assay Details
Assay
Reference: J:311886 Varghese PC, et al., Histone chaperone APLF level dictates the implantation of mouse embryos. J Cell Sci. 2021 Jan 13;134(1):jcs246900
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6885855
Gene symbol: Aplf
Gene name: aprataxin and PNKP like factor
Antibody: anti-APLF
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A: embryonic day 2.0 (more )
Note: Double labeled: green - Aplf; red - Cdh1 (assay MGI:6885856).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 1A APLF , 1A merge Expressed within the nucleus, as well as in the cytosol.
Specimen 1B upper: embryonic day 3.0 (more )
Note: 16-cell stage. Double labeled: green - Aplf; red - Cdh1 (assay MGI:6885856).
Structure Level Pattern Image Note
TS4: compacted morula Present 1B upper APLF , 1B upper merge Enhanced expression.
Specimen 1B lower: embryonic day 3.0 (more )
Note: Morula stage. Double labeled: green - Aplf; red - Cdh1 (assay MGI:6885856).
Structure Level Pattern Image Note
TS4: compacted morula Present 1B lower APLF , 1B lower merge Enhanced expression.
Specimen 1C: embryonic day 3.5 (more )
Note: Double labeled: green - Aplf; red - Cdh1 (assay MGI:6885856).
Structure Level Pattern Image Note
TS4: blastocyst Present 1C APLF , 1C merge Significantly downregulated expression. A significant level of expression was enhanced within the trophectoderm.
TS4: trophectoderm Present 1C APLF , 1C merge
Specimen 1E: embryonic day 3.5 (more )
Note: Double labeled: green - Aplf; red - Tead4 (assay MGI:6885858).
Structure Level Pattern Image Note
TS4: blastocyst Present 1E APLF , 1E merge Significantly downregulated expression. A significant level of expression was enhanced within the trophectoderm.
TS4: trophectoderm Present 1E merge , 1E APLF
Specimen 2F/H/I: embryonic day 7.5 (more )
Note: Double labeled: green - Aplf; red - Cdh1 (assay MGI:6885856).
Structure Level Pattern Image Note
TS11: trophectoderm Present 2H , 2I , 2F
TS11: trophoblast giant cell Present 2H , 2I , 2F
TS11: allantois Present 2F , 2H
TS11: ectoplacental cone Present 2F , 2H
Specimen S1A: embryonic day 2.0 (more )
Note: Double labeled: green - Aplf; red - Cdh1 (assay MGI:6885856).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present S1A APLF , S1A merge Expressed within the nucleus, as well as in the cytosol.
Specimen S1B: embryonic day 3.0 (more )
Note: Early morula. Double labeled: green - Aplf; red - Cdh1 (assay MGI:6885856).
Structure Level Pattern Image Note
TS4: compacted morula Present S1B APLF , S1B merge Enhanced expression.
Specimen S1C: embryonic day 3.5 (more )
Note: Early blastocyst. Double labeled: green - Aplf; red - Cdh1 (assay MGI:6885856).
Structure Level Pattern Image Note
TS4: blastocyst Present S1C APLF , S1C merge
Specimen S1D: embryonic day 3.5 (more )
Note: Double labeled: green - Aplf; red - Pou5f1 (assay MGI:6885857).
Structure Level Pattern Image Note
TS4: blastocyst Present S1D APLF , S1D merge Significantly downregulated expression. A significant level of expression was enhanced within the trophectoderm.
TS4: trophectoderm Present S1D merge , S1D APLF
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory