Antibody:	anti-Osterix (ab94744)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present	Uniform	6A Ankrd11ctrl Sp7

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present	Graded	6A Ankrd11ncko Sp7	Expression is strong in the center and becomes diffuse toward the periphery. Quantification of the expression domains indicated that expression was more contained, but the differences in the area occupied did not reach statistical significance.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: maxilla	osteoblast	Present		6C Ankrd11ctrl Sp7 left , 6C Ankrd11ctrl Sp7 right	Expression is in osteoblasts lining the newly formed trabeculae.

Structure	Level	Pattern	Image	Note
TS27: maxilla	Present	Regionally restricted	6C Ankrd11ncko Sp7 left , 6C Ankrd11ncko Sp7 right	Expression is patchy and more restricted to the small sparse trabeculae. In this mutant the number nor area of positive cells were significantly different compared to controls.

Structure	Level	Pattern	Image	Note
TS22: secondary palate	Present		7B Sp7 E14.5

Structure	Level	Pattern	Image	Note
TS27: secondary palate	Present		7B Sp7 P0